6KBB

Role of the DEF/Y motif of Swc5 in histone H2A.Z deposition

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.987
Spacegroup name	C 1 2 1
Unit cell lengths	107.072, 65.521, 84.821
Unit cell angles	90.00, 122.83, 90.00

Refinement procedure

Resolution	44.986 - 2.365
R-factor	0.1894
Rwork	0.188
R-free	0.22560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2cv5
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.15_3459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.365	2.365
Rmerge	0.150	0.953
Number of reflections	20109	1999
<I/σ(I)>	12.235
Completeness [%]	99.9
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	0.1M sodium acetate tribasic dihydrate, pH 5.3, 19.5% PEG 1000