6K4S

Crystal structure of xCas9 in complex with sgRNA and DNA (TGC PAM)

Summary for 6K4S

Entry DOI	10.2210/pdb6k4s/pdb
Descriptor	CRISPR-associated endonuclease Cas9/Csn1, sgRNA, targeted DNA, ... (4 entities in total)
Functional Keywords	endonuclease, hydrolase-dna-rna complex, hydrolase/dna/rna
Biological source	Streptococcus pyogenes serotype M1 More
Total number of polymer chains	4
Total formula weight	197641.86
Authors	Chen, W.,Zhang, H.,Zhang, Y.,Wang, Y.,Gan, J.,Ji, Q. (deposition date: 2019-05-26, release date: 2019-09-25, Last modification date: 2023-11-22)
Primary citation	Chen, W.,Zhang, H.,Zhang, Y.,Wang, Y.,Gan, J.,Ji, Q. Molecular basis for the PAM expansion and fidelity enhancement of an evolved Cas9 nuclease. Plos Biol., 17:e3000496-e3000496, 2019 Cited by PubMed: 31603896 DOI: 10.1371/journal.pbio.3000496 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (3.01 Å)

Structure validation