6K4S
Crystal structure of xCas9 in complex with sgRNA and DNA (TGC PAM)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 85 |
Detector technology | CCD |
Collection date | 2018-09-21 |
Detector | AGILENT EOS CCD |
Wavelength(s) | 0.9785 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 177.464, 70.364, 188.899 |
Unit cell angles | 90.00, 110.42, 90.00 |
Refinement procedure
Resolution | 49.250 - 3.010 |
R-factor | 0.23934 |
Rwork | 0.237 |
R-free | 0.28531 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4un3 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.659 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rpim | 0.071 | 0.578 |
Number of reflections | 43368 | 4176 |
<I/σ(I)> | 12 | |
Completeness [%] | 98.8 | 96.9 |
Redundancy | 6.5 | 5.5 |
CC(1/2) | 1.000 | 0.543 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289 | 0.2 M Ammonium phosphate dibasic, 20% w/v Polyethylene glycol 3,350 |