6K4Q

Crystal structure of xCas9 in complex with sgRNA and DNA (CGG PAM)

Summary for 6K4Q

• Entry DOI: 10.2210/pdb6k4q/pdb
• Descriptor: sgRNA, DNA (28-MER), DNA (5'-D(*AP*AP*AP*CP*GP*GP*TP*AP*TP*TP*G)-3'), ... (6 entities in total)
• Functional Keywords: endonuclease, hydrolase-dna-rna complex, hydrolase/dna/rna
• Biological source: Streptococcus pyogenes serotype M1; More
• Total number of polymer chains: 4
• Total formula weight: 198497.58

Authors

Chen, W.,Zhang, H.,Zhang, Y.,Wang, Y.,Gan, J.,Ji, Q. (deposition date: 2019-05-26, release date: 2019-09-25, Last modification date: 2023-11-22)

• Primary citation: Chen, W.,Zhang, H.,Zhang, Y.,Wang, Y.,Gan, J.,Ji, Q.; Molecular basis for the PAM expansion and fidelity enhancement of an evolved Cas9 nuclease.; Plos Biol., 17:e3000496-e3000496, 2019; PubMed: 31603896; DOI: 10.1371/journal.pbio.3000496

Experimental method

X-RAY DIFFRACTION (2.7 Å)