6K4Q
Crystal structure of xCas9 in complex with sgRNA and DNA (CGG PAM)
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | sgRNA | polymer | 83 | 26789.0 | 1 | Streptococcus pyogenes | ||
2 | C | DNA (28-MER) | polymer | 28 | 8568.6 | 1 | Streptococcus pyogenes | ||
3 | D | DNA (5'-D(*AP*AP*AP*CP*GP*GP*TP*AP*TP*TP*G)-3') | polymer | 12 | 3710.5 | 1 | Streptococcus pyogenes | ||
4 | B | CRISPR-associated endonuclease Cas9/Csn1 | polymer | 1368 | 158574.8 | 1 | UniProt (Q99ZW2) Pfam (PF16593) Pfam (PF16592) Pfam (PF13395) Pfam (PF22702) Pfam (PF16595) In PDB | Streptococcus pyogenes serotype M1 | SpCas9,SpyCas9 |
5 | A, B | PHOSPHATE ION | non-polymer | 95.0 | 9 | Chemie (PO4) | |||
6 | water | water | 18.0 | 99 | Chemie (HOH) |
Sequence modifications
B: 1 - 1368 (UniProt: Q99ZW2)
PDB | External Database | Details |
---|---|---|
Ala 10 | Asp 10 | engineered mutation |
Leu 80 | Cys 80 | engineered mutation |
Thr 262 | Ala 262 | engineered mutation |
Leu 324 | Arg 324 | engineered mutation |
Ile 409 | Ser 409 | engineered mutation |
Lys 480 | Glu 480 | engineered mutation |
Asp 543 | Glu 543 | engineered mutation |
Glu 574 | Cys 574 | engineered mutation |
Ile 694 | Met 694 | engineered mutation |
Ala 840 | His 840 | engineered mutation |
Val 1219 | Glu 1219 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 197642.8 | |
Non-Polymers* | Number of molecules | 9 |
Total formula weight | 854.7 | |
All* | Total formula weight | 198497.6 |