6JQ6
Hatchet Ribozyme Structure soaking with Ir(NH3)6+
Summary for 6JQ6
Entry DOI | 10.2210/pdb6jq6/pdb |
Descriptor | RNA (81-MER), IRIDIUM HEXAMMINE ION (3 entities in total) |
Functional Keywords | ribozyme, hatchet, cleavage, catalysis, non-coding rna, rna |
Biological source | synthetic construct |
Total number of polymer chains | 1 |
Total formula weight | 26299.78 |
Authors | |
Primary citation | Zheng, L.,Falschlunger, C.,Huang, K.,Mairhofer, E.,Yuan, S.,Wang, J.,Patel, D.J.,Micura, R.,Ren, A. Hatchet ribozyme structure and implications for cleavage mechanism. Proc.Natl.Acad.Sci.USA, 116:10783-10791, 2019 Cited by PubMed Abstract: Small self-cleaving ribozymes catalyze site-specific cleavage of their own phosphodiester backbone with implications for viral genome replication, pre-mRNA processing, and alternative splicing. We report on the 2.1-Å crystal structure of the hatchet ribozyme product, which adopts a compact pseudosymmetric dimeric scaffold, with each monomer stabilized by long-range interactions involving highly conserved nucleotides brought into close proximity of the scissile phosphate. Strikingly, the catalytic pocket contains a cavity capable of accommodating both the modeled scissile phosphate and its flanking 5' nucleoside. The resulting modeled precatalytic conformation incorporates a splayed-apart alignment at the scissile phosphate, thereby providing structure-based insights into the in-line cleavage mechanism. We identify a guanine lining the catalytic pocket positioned to contribute to cleavage chemistry. The functional relevance of structure-based insights into hatchet ribozyme catalysis is strongly supported by cleavage assays monitoring the impact of selected nucleobase and atom-specific mutations on ribozyme activity. PubMed: 31088965DOI: 10.1073/pnas.1902413116 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (2.626 Å) |
Structure validation
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