6JQ6

Hatchet Ribozyme Structure soaking with Ir(NH3)6+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-02
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.103
Spacegroup name	P 63 2 2
Unit cell lengths	91.830, 91.830, 140.627
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.626
R-factor	0.1996
Rwork	0.196
R-free	0.22930
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.236
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.720
High resolution limit [Å]	2.610	2.610
Rpim	0.041	2.290
Number of reflections	19049	683
<I/σ(I)>	20.2
Completeness [%]	99.0
Redundancy	20

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.20M K-formate, 2.2M (NH4)2SO4