6JPS

Human H chain ferritin mutant-MBP

Summary for 6JPS

• Entry DOI: 10.2210/pdb6jps/pdb
• Descriptor: Ferritin (1 entity in total)
• Functional Keywords: ferritin, mercuric ion binding peptide, metal binding protein
• Biological source: Homo sapiens (Human)
• Total number of polymer chains: 24
• Total formula weight: 522268.87

Authors

Wang, Y.,Zang, J.,Chen, H. (deposition date: 2019-03-27, release date: 2019-10-09, Last modification date: 2023-11-22)

Primary citation

Wang, Y.,Chen, H.,Zang, J.,Zhang, X.,Zhao, G.
Re-designing ferritin nanocages for mercuric ion detection.
Analyst, 144:5890-5897, 2019

PubMed Abstract: Protein nanocages have recently received considerable attention in the fields of nanoscience and nanomedicine and have been used as either biotemplates for the preparation of a variety of nanomaterials or vehicles for drugs or imaging agents. However, their utilization for detection of heavy metal ions has yet to be explored. In this study, by grafting a mercury binding peptide (MBP) on the exterior surface of a recombinant human H-chain ferritin (rHuHF) nanocage, we successfully prepared a new protein nanocage (HuHF-MBP) which exhibits high binding capacity and affinity for Hg2+. The fluorescence of HuHF-MBP labeled with a green fluorescent dye fluorescein isothiocyanate (FITC) can be quenched by graphene oxide (GO), while addition of Hg2+ to the above solution recovered the quenched fluorescence in a dose-dependent manner. Thus, this system consisting of FITC-labeled HuHF-MBP and GO, where FITC and graphene oxide were used as fluorescent reporter probes, has great potential to be explored as a sensor for Hg2+ detection. Indeed, this newly constructed protein sensor exhibited high sensitivity and selectivity for Hg2+, and the limit of detection was 1.0 nM. The construction of this system provides an alternative strategy for the preparation of heavy metal ion sensors by using protein nanocages as biotemplates.

PubMed: 31497803
DOI: 10.1039/c9an01110b

Experimental method

X-RAY DIFFRACTION (3.503 Å)