6JPS
Human H chain ferritin mutant-MBP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 291 |
Detector technology | CCD |
Collection date | 2018-04-03 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9789 |
Spacegroup name | P 42 |
Unit cell lengths | 217.524, 217.524, 146.821 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.750 - 3.503 |
R-factor | 0.3409 |
Rwork | 0.339 |
R-free | 0.39870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fha |
RMSD bond length | 0.003 |
RMSD bond angle | 0.482 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.750 | 3.628 |
High resolution limit [Å] | 3.503 | 3.503 |
Number of reflections | 85322 | |
<I/σ(I)> | 1.5 | |
Completeness [%] | 98.0 | |
Redundancy | 1 | |
CC(1/2) | 0.945 | 0.945 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | magnesium chloride, Tris, 1,6-hexanediol |