6JPS

Human H chain ferritin mutant-MBP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	291
Detector technology	CCD
Collection date	2018-04-03
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9789
Spacegroup name	P 42
Unit cell lengths	217.524, 217.524, 146.821
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.750 - 3.503
R-factor	0.3409
Rwork	0.339
R-free	0.39870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fha
RMSD bond length	0.003
RMSD bond angle	0.482
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	HKL-3000
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.750	3.628
High resolution limit [Å]	3.503	3.503
Number of reflections	85322
<I/σ(I)>	1.5
Completeness [%]	98.0
Redundancy	1
CC(1/2)	0.945	0.945

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	magnesium chloride, Tris, 1,6-hexanediol