6JOO
Crystal structure of Corynebacterium diphtheriae Cas9 in complex with sgRNA and target DNA
Summary for 6JOO
Entry DOI | 10.2210/pdb6joo/pdb |
Descriptor | CRISPR-associated protein,CRISPR-associated endonuclease Cas9, Guide RNA, Non-Target DNA, ... (7 entities in total) |
Functional Keywords | crispr-cas, cas9, pam, dna endonuclease, ribonucleoprotein, hydrolase-dna-rna complex, hydrolase/dna/rna |
Biological source | Corynebacterium diphtheriae More |
Total number of polymer chains | 4 |
Total formula weight | 151414.24 |
Authors | Hirano, S.,Ishitani, R.,Nishimasu, H.,Nureki, O. (deposition date: 2019-03-22, release date: 2019-04-17, Last modification date: 2019-05-15) |
Primary citation | Hirano, S.,Abudayyeh, O.O.,Gootenberg, J.S.,Horii, T.,Ishitani, R.,Hatada, I.,Zhang, F.,Nishimasu, H.,Nureki, O. Structural basis for the promiscuous PAM recognition by Corynebacterium diphtheriae Cas9. Nat Commun, 10:1968-1968, 2019 Cited by PubMed: 31036811DOI: 10.1038/s41467-019-09741-6 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (2.9 Å) |
Structure validation
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