6JOO

Crystal structure of Corynebacterium diphtheriae Cas9 in complex with sgRNA and target DNA

Summary for 6JOO

Entry DOI	10.2210/pdb6joo/pdb
Descriptor	CRISPR-associated protein,CRISPR-associated endonuclease Cas9, Guide RNA, Non-Target DNA, ... (7 entities in total)
Functional Keywords	crispr-cas, cas9, pam, dna endonuclease, ribonucleoprotein, hydrolase-dna-rna complex, hydrolase/dna/rna
Biological source	Corynebacterium diphtheriae More
Total number of polymer chains	4
Total formula weight	151414.24
Authors	Hirano, S.,Ishitani, R.,Nishimasu, H.,Nureki, O. (deposition date: 2019-03-22, release date: 2019-04-17, Last modification date: 2019-05-15)
Primary citation	Hirano, S.,Abudayyeh, O.O.,Gootenberg, J.S.,Horii, T.,Ishitani, R.,Hatada, I.,Zhang, F.,Nishimasu, H.,Nureki, O. Structural basis for the promiscuous PAM recognition by Corynebacterium diphtheriae Cas9. Nat Commun, 10:1968-1968, 2019 Cited by PubMed: 31036811 DOI: 10.1038/s41467-019-09741-6 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.9 Å)

Structure validation