6JOO
Crystal structure of Corynebacterium diphtheriae Cas9 in complex with sgRNA and target DNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | CRISPR-associated protein,CRISPR-associated endonuclease Cas9 | polymer | 927 | 103966.2 | 1 | UniProt (A0A2T1BQ76) UniProt (Q6NKI3) Pfam (PF18470) Pfam (PF18541) Pfam (PF17893) Pfam (PF17894) Pfam (PF18525) | Corynebacterium diphtheriae | SaCas9 |
| 2 | B (B) | Guide RNA | polymer | 113 | 36321.4 | 1 | Corynebacterium diphtheriae | ||
| 3 | C (D) | Non-Target DNA | polymer | 8 | 2506.7 | 1 | Corynebacterium diphtheriae | ||
| 4 | D (C) | Target DNA | polymer | 28 | 8430.4 | 1 | Corynebacterium diphtheriae | ||
| 5 | E, G (A, B) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 2 | Chemie (EDO) | |||
| 6 | F (A) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
| 7 | H (A) | water | water | 18.0 | 3 | Chemie (HOH) |
Sequence modifications
A: 1 - 497 (UniProt: A0A2T1BQ76)
A: 664 - 1084 (UniProt: Q6NKI3)
| PDB | External Database | Details |
|---|---|---|
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| His 0 | - | expression tag |
| Gly 658 | - | linker |
| Gly 659 | - | linker |
| Gly 660 | - | linker |
| Ser 661 | - | linker |
| Gly 662 | - | linker |
| Gly 663 | - | linker |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 151224.7 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 189.5 | |
| All* | Total formula weight | 151414.2 |
