cryo-EM structure of DOT1L bound to unmodified nucleosome

Summary for 6JM9

EMDB information9843 9844
DescriptorDNA strand I, DNA strand J, Histone H3.2, ... (8 entities in total)
Functional Keywordshistone, nucleosome, methylation, gene regulation
Biological sourcesynthetic construct
Total number of polymer chains11
Total molecular weight201592.86
Jang, S.,Song, J.J. (deposition date: 2019-03-07, release date: 2019-05-15, Last modification date: 2019-05-22)
Primary citation
Jang, S.,Kang, C.,Yang, H.S.,Jung, T.,Hebert, H.,Chung, K.Y.,Kim, S.J.,Hohng, S.,Song, J.J.
Structural basis of recognition and destabilization of the histone H2B ubiquitinated nucleosome by the DOT1L histone H3 Lys79 methyltransferase.
Genes Dev., 2019
PubMed: 30923167 (PDB entries with the same primary citation)
DOI: 10.1101/gad.323790.118
MImport into Mendeley
Experimental method

Structure validation

ClashscoreRamachandran outliersSidechain outliers13 1.3% 3.2%MetricValuePercentile RanksWorseBetterPercentile relative to all structuresPercentile relative to all EM structures

More Asymmetric unit images

Molmil generated image of 6jm9
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Molmil generated image of 6jm9
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Molmil generated image of 6jm9
rotated about y axis by 90°