6JE3

Crystal structure of Nme2Cas9 in complex with sgRNA and target DNA (AGGCCC PAM) with 5 nt overhang

Summary for 6JE3

Entry DOI	10.2210/pdb6je3/pdb
Descriptor	CRISPR-associated endonuclease Cas9, sgRNA, target DNA strand, ... (6 entities in total)
Functional Keywords	crispr-cas9, nmecas9, nme2cas9, hydrolase-rna-dna complex, hydrolase/rna/dna
Biological source	Neisseria meningitidis More
Total number of polymer chains	4
Total formula weight	183810.79
Authors	Sun, W.,Yang, J.,Cheng, Z.,Liu, C.,Wang, K.,Huang, X.,Wang, Y. (deposition date: 2019-02-03, release date: 2019-11-06, Last modification date: 2023-11-22)
Primary citation	Sun, W.,Yang, J.,Cheng, Z.,Amrani, N.,Liu, C.,Wang, K.,Ibraheim, R.,Edraki, A.,Huang, X.,Wang, M.,Wang, J.,Liu, L.,Sheng, G.,Yang, Y.,Lou, J.,Sontheimer, E.J.,Wang, Y. Structures of Neisseria meningitidis Cas9 Complexes in Catalytically Poised and Anti-CRISPR-Inhibited States. Mol.Cell, 76:938-, 2019 Cited by PubMed: 31668930 DOI: 10.1016/j.molcel.2019.09.025 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.931 Å)

Structure validation