6JE3
Crystal structure of Nme2Cas9 in complex with sgRNA and target DNA (AGGCCC PAM) with 5 nt overhang
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | CRISPR-associated endonuclease Cas9 | polymer | 1083 | 124913.1 | 1 | Neisseria meningitidis | ||
| 2 | B (B) | sgRNA | polymer | 135 | 43059.4 | 1 | synthetic construct | ||
| 3 | C (C) | target DNA strand | polymer | 35 | 10845.0 | 1 | synthetic construct | ||
| 4 | D (D) | non-target DNA strand | polymer | 16 | 4931.2 | 1 | synthetic construct | ||
| 5 | E (A) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 1 | Chemie (EDO) | |||
| 6 | F, G, H (A, B, C) | water | water | 18.0 | 15 | Chemie (HOH) |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 183748.7 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 62.1 | |
| All* | Total formula weight | 183810.8 |
