6JCZ

Cryo-EM Structure of Sulfolobus solfataricus ketol-acid reductoisomerase (Sso-KARI) in complex with Mg2+, NADPH, and CPD at pH7.5

Summary for 6JCZ

Entry DOI	10.2210/pdb6jcz/pdb
EMDB information	9800
Descriptor	Putative ketol-acid reductoisomerase 2, MAGNESIUM ION, NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, ... (5 entities in total)
Functional Keywords	bi-specific, thermostable, reductoisomerase, magnesium-dependent, dodecamer, knotted protein, isomerase
Biological source	Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus)
Total number of polymer chains	12
Total formula weight	457847.82
Authors	Chen, C.Y.,Chang, Y.C.,Lin, K.F.,Huang, C.H.,Lin, B.L.,Ko, T.P.,Hsieh, D.L.,Tsai, M.D. (deposition date: 2019-01-30, release date: 2019-04-17, Last modification date: 2019-05-01)
Primary citation	Chen, C.Y.,Chang, Y.C.,Lin, B.L.,Lin, K.F.,Huang, C.H.,Hsieh, D.L.,Ko, T.P.,Tsai, M.D. Use of Cryo-EM To Uncover Structural Bases of pH Effect and Cofactor Bispecificity of Ketol-Acid Reductoisomerase. J. Am. Chem. Soc., 141:6136-6140, 2019 Cited by PubMed: 30921515 DOI: 10.1021/jacs.9b01354 PDB entries with the same primary citation
Experimental method	ELECTRON MICROSCOPY (3.35 Å)

Structure validation