6JCZ
Cryo-EM Structure of Sulfolobus solfataricus ketol-acid reductoisomerase (Sso-KARI) in complex with Mg2+, NADPH, and CPD at pH7.5
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004455 | molecular_function | ketol-acid reductoisomerase activity |
| A | 0008652 | biological_process | amino acid biosynthetic process |
| A | 0009082 | biological_process | branched-chain amino acid biosynthetic process |
| A | 0009097 | biological_process | isoleucine biosynthetic process |
| A | 0009099 | biological_process | valine biosynthetic process |
| A | 0016491 | molecular_function | oxidoreductase activity |
| B | 0004455 | molecular_function | ketol-acid reductoisomerase activity |
| B | 0008652 | biological_process | amino acid biosynthetic process |
| B | 0009082 | biological_process | branched-chain amino acid biosynthetic process |
| B | 0009097 | biological_process | isoleucine biosynthetic process |
| B | 0009099 | biological_process | valine biosynthetic process |
| B | 0016491 | molecular_function | oxidoreductase activity |
| C | 0004455 | molecular_function | ketol-acid reductoisomerase activity |
| C | 0008652 | biological_process | amino acid biosynthetic process |
| C | 0009082 | biological_process | branched-chain amino acid biosynthetic process |
| C | 0009097 | biological_process | isoleucine biosynthetic process |
| C | 0009099 | biological_process | valine biosynthetic process |
| C | 0016491 | molecular_function | oxidoreductase activity |
| D | 0004455 | molecular_function | ketol-acid reductoisomerase activity |
| D | 0008652 | biological_process | amino acid biosynthetic process |
| D | 0009082 | biological_process | branched-chain amino acid biosynthetic process |
| D | 0009097 | biological_process | isoleucine biosynthetic process |
| D | 0009099 | biological_process | valine biosynthetic process |
| D | 0016491 | molecular_function | oxidoreductase activity |
| E | 0004455 | molecular_function | ketol-acid reductoisomerase activity |
| E | 0008652 | biological_process | amino acid biosynthetic process |
| E | 0009082 | biological_process | branched-chain amino acid biosynthetic process |
| E | 0009097 | biological_process | isoleucine biosynthetic process |
| E | 0009099 | biological_process | valine biosynthetic process |
| E | 0016491 | molecular_function | oxidoreductase activity |
| F | 0004455 | molecular_function | ketol-acid reductoisomerase activity |
| F | 0008652 | biological_process | amino acid biosynthetic process |
| F | 0009082 | biological_process | branched-chain amino acid biosynthetic process |
| F | 0009097 | biological_process | isoleucine biosynthetic process |
| F | 0009099 | biological_process | valine biosynthetic process |
| F | 0016491 | molecular_function | oxidoreductase activity |
| G | 0004455 | molecular_function | ketol-acid reductoisomerase activity |
| G | 0008652 | biological_process | amino acid biosynthetic process |
| G | 0009082 | biological_process | branched-chain amino acid biosynthetic process |
| G | 0009097 | biological_process | isoleucine biosynthetic process |
| G | 0009099 | biological_process | valine biosynthetic process |
| G | 0016491 | molecular_function | oxidoreductase activity |
| H | 0004455 | molecular_function | ketol-acid reductoisomerase activity |
| H | 0008652 | biological_process | amino acid biosynthetic process |
| H | 0009082 | biological_process | branched-chain amino acid biosynthetic process |
| H | 0009097 | biological_process | isoleucine biosynthetic process |
| H | 0009099 | biological_process | valine biosynthetic process |
| H | 0016491 | molecular_function | oxidoreductase activity |
| I | 0004455 | molecular_function | ketol-acid reductoisomerase activity |
| I | 0008652 | biological_process | amino acid biosynthetic process |
| I | 0009082 | biological_process | branched-chain amino acid biosynthetic process |
| I | 0009097 | biological_process | isoleucine biosynthetic process |
| I | 0009099 | biological_process | valine biosynthetic process |
| I | 0016491 | molecular_function | oxidoreductase activity |
| J | 0004455 | molecular_function | ketol-acid reductoisomerase activity |
| J | 0008652 | biological_process | amino acid biosynthetic process |
| J | 0009082 | biological_process | branched-chain amino acid biosynthetic process |
| J | 0009097 | biological_process | isoleucine biosynthetic process |
| J | 0009099 | biological_process | valine biosynthetic process |
| J | 0016491 | molecular_function | oxidoreductase activity |
| K | 0004455 | molecular_function | ketol-acid reductoisomerase activity |
| K | 0008652 | biological_process | amino acid biosynthetic process |
| K | 0009082 | biological_process | branched-chain amino acid biosynthetic process |
| K | 0009097 | biological_process | isoleucine biosynthetic process |
| K | 0009099 | biological_process | valine biosynthetic process |
| K | 0016491 | molecular_function | oxidoreductase activity |
| L | 0004455 | molecular_function | ketol-acid reductoisomerase activity |
| L | 0008652 | biological_process | amino acid biosynthetic process |
| L | 0009082 | biological_process | branched-chain amino acid biosynthetic process |
| L | 0009097 | biological_process | isoleucine biosynthetic process |
| L | 0009099 | biological_process | valine biosynthetic process |
| L | 0016491 | molecular_function | oxidoreductase activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 4 |
| Details | binding site for residue MG A 401 |
| Chain | Residue |
| A | ASP191 |
| A | GLU195 |
| A | 9TY404 |
| B | SER257 |
| site_id | AC2 |
| Number of Residues | 3 |
| Details | binding site for residue MG A 402 |
| Chain | Residue |
| A | SER194 |
| A | 9TY404 |
| B | GLU227 |
| site_id | AC3 |
| Number of Residues | 13 |
| Details | binding site for residue NDP A 403 |
| Chain | Residue |
| A | GLY24 |
| A | ASN25 |
| A | GLN26 |
| A | ASN45 |
| A | VAL46 |
| A | ASP48 |
| A | TYR50 |
| A | ILE78 |
| A | VAL82 |
| A | SER107 |
| A | 9TY404 |
| A | GLY22 |
| A | TYR23 |
| site_id | AC4 |
| Number of Residues | 6 |
| Details | binding site for residue 9TY A 404 |
| Chain | Residue |
| A | ASP191 |
| A | GLU195 |
| A | MG401 |
| A | MG402 |
| A | NDP403 |
| B | SER257 |
| site_id | AC5 |
| Number of Residues | 2 |
| Details | binding site for residue MG A 405 |
| Chain | Residue |
| A | GLU227 |
| B | SER194 |
| site_id | AC6 |
| Number of Residues | 3 |
| Details | binding site for residue MG B 401 |
| Chain | Residue |
| B | ASP191 |
| B | GLU195 |
| B | 9TY403 |
| site_id | AC7 |
| Number of Residues | 13 |
| Details | binding site for residue NDP B 402 |
| Chain | Residue |
| B | GLY22 |
| B | TYR23 |
| B | GLY24 |
| B | ASN25 |
| B | GLN26 |
| B | ASN45 |
| B | VAL46 |
| B | TYR50 |
| B | ILE78 |
| B | PRO79 |
| B | SER107 |
| B | PRO129 |
| B | 9TY403 |
| site_id | AC8 |
| Number of Residues | 7 |
| Details | binding site for residue 9TY B 403 |
| Chain | Residue |
| A | SER257 |
| B | SER107 |
| B | ASP191 |
| B | GLU195 |
| B | MG401 |
| B | NDP402 |
| B | HOH505 |
| site_id | AC9 |
| Number of Residues | 3 |
| Details | binding site for residue MG C 401 |
| Chain | Residue |
| C | ASP191 |
| C | GLU195 |
| C | 9TY403 |
| site_id | AD1 |
| Number of Residues | 12 |
| Details | binding site for residue NDP C 402 |
| Chain | Residue |
| C | TYR23 |
| C | GLY24 |
| C | ASN25 |
| C | GLN26 |
| C | ASN45 |
| C | VAL46 |
| C | ILE78 |
| C | VAL82 |
| C | SER107 |
| C | PRO129 |
| C | VAL132 |
| C | 9TY403 |
| site_id | AD2 |
| Number of Residues | 8 |
| Details | binding site for residue 9TY C 403 |
| Chain | Residue |
| C | ASP191 |
| C | GLU195 |
| C | MG401 |
| C | NDP402 |
| C | HOH502 |
| D | SER254 |
| D | SER257 |
| D | MG401 |
| site_id | AD3 |
| Number of Residues | 4 |
| Details | binding site for residue MG C 404 |
| Chain | Residue |
| C | GLU227 |
| C | GLU233 |
| D | SER194 |
| D | 9TY404 |
| site_id | AD4 |
| Number of Residues | 3 |
| Details | binding site for residue MG D 401 |
| Chain | Residue |
| C | SER194 |
| C | 9TY403 |
| D | GLU227 |
| site_id | AD5 |
| Number of Residues | 4 |
| Details | binding site for residue MG D 402 |
| Chain | Residue |
| C | SER257 |
| D | ASP191 |
| D | GLU195 |
| D | 9TY404 |
| site_id | AD6 |
| Number of Residues | 13 |
| Details | binding site for residue NDP D 403 |
| Chain | Residue |
| D | TYR23 |
| D | GLY24 |
| D | ASN25 |
| D | GLN26 |
| D | ASN45 |
| D | VAL46 |
| D | TYR50 |
| D | ILE78 |
| D | PRO79 |
| D | SER107 |
| D | PRO129 |
| D | VAL132 |
| D | 9TY404 |
| site_id | AD7 |
| Number of Residues | 7 |
| Details | binding site for residue 9TY D 404 |
| Chain | Residue |
| C | SER257 |
| C | MG404 |
| D | ASP191 |
| D | GLU195 |
| D | MG402 |
| D | NDP403 |
| C | GLU233 |
| site_id | AD8 |
| Number of Residues | 4 |
| Details | binding site for residue MG E 401 |
| Chain | Residue |
| E | ASP191 |
| E | GLU195 |
| E | 9TY403 |
| F | SER257 |
| site_id | AD9 |
| Number of Residues | 15 |
| Details | binding site for residue NDP E 402 |
| Chain | Residue |
| E | TYR23 |
| E | GLY24 |
| E | ASN25 |
| E | GLN26 |
| E | ASN45 |
| E | VAL46 |
| E | ASP48 |
| E | TYR50 |
| E | ILE78 |
| E | PRO79 |
| E | VAL82 |
| E | SER107 |
| E | PRO129 |
| E | VAL132 |
| E | 9TY403 |
| site_id | AE1 |
| Number of Residues | 6 |
| Details | binding site for residue 9TY E 403 |
| Chain | Residue |
| E | ASP191 |
| E | GLU195 |
| E | MG401 |
| E | NDP402 |
| E | MG404 |
| F | SER257 |
| site_id | AE2 |
| Number of Residues | 2 |
| Details | binding site for residue MG E 404 |
| Chain | Residue |
| E | 9TY403 |
| F | GLU227 |
| site_id | AE3 |
| Number of Residues | 4 |
| Details | binding site for residue MG F 401 |
| Chain | Residue |
| E | GLU227 |
| E | GLU233 |
| F | SER194 |
| F | 9TY404 |
| site_id | AE4 |
| Number of Residues | 3 |
| Details | binding site for residue MG F 402 |
| Chain | Residue |
| F | ASP191 |
| F | GLU195 |
| F | 9TY404 |
| site_id | AE5 |
| Number of Residues | 12 |
| Details | binding site for residue NDP F 403 |
| Chain | Residue |
| F | TYR23 |
| F | GLY24 |
| F | ASN25 |
| F | GLN26 |
| F | ASN45 |
| F | VAL46 |
| F | TYR50 |
| F | ILE78 |
| F | VAL82 |
| F | ALA106 |
| F | SER107 |
| F | 9TY404 |
| site_id | AE6 |
| Number of Residues | 7 |
| Details | binding site for residue 9TY F 404 |
| Chain | Residue |
| E | SER257 |
| F | ARG130 |
| F | ASP191 |
| F | GLU195 |
| F | MG401 |
| F | MG402 |
| F | NDP403 |
| site_id | AE7 |
| Number of Residues | 3 |
| Details | binding site for residue MG G 401 |
| Chain | Residue |
| G | ASP191 |
| G | GLU195 |
| G | 9TY403 |
| site_id | AE8 |
| Number of Residues | 8 |
| Details | binding site for residue 9TY G 403 |
| Chain | Residue |
| G | ARG130 |
| G | ASP191 |
| G | GLU195 |
| G | MG401 |
| G | NDP402 |
| H | GLU227 |
| H | SER257 |
| H | MG402 |
| site_id | AE9 |
| Number of Residues | 3 |
| Details | binding site for residue MG H 401 |
| Chain | Residue |
| G | GLU227 |
| G | GLU233 |
| H | 9TY405 |
| site_id | AF1 |
| Number of Residues | 5 |
| Details | binding site for residue MG H 402 |
| Chain | Residue |
| G | SER194 |
| G | GLU195 |
| G | 9TY403 |
| H | GLU227 |
| H | GLU233 |
| site_id | AF2 |
| Number of Residues | 4 |
| Details | binding site for residue MG H 403 |
| Chain | Residue |
| G | SER257 |
| H | ASP191 |
| H | GLU195 |
| H | 9TY405 |
| site_id | AF3 |
| Number of Residues | 12 |
| Details | binding site for residue NDP H 404 |
| Chain | Residue |
| H | TYR23 |
| H | GLY24 |
| H | ASN25 |
| H | GLN26 |
| H | ASN45 |
| H | VAL46 |
| H | ILE78 |
| H | VAL82 |
| H | SER107 |
| H | PRO129 |
| H | VAL132 |
| H | 9TY405 |
| site_id | AF4 |
| Number of Residues | 7 |
| Details | binding site for residue 9TY H 405 |
| Chain | Residue |
| G | SER254 |
| G | SER257 |
| H | ASP191 |
| H | GLU195 |
| H | MG401 |
| H | MG403 |
| H | NDP404 |
| site_id | AF5 |
| Number of Residues | 4 |
| Details | binding site for residue MG I 401 |
| Chain | Residue |
| I | ASP191 |
| I | GLU195 |
| I | 9TY403 |
| J | SER257 |
| site_id | AF6 |
| Number of Residues | 14 |
| Details | binding site for residue NDP I 402 |
| Chain | Residue |
| I | TYR23 |
| I | GLY24 |
| I | ASN25 |
| I | GLN26 |
| I | ASN45 |
| I | VAL46 |
| I | TYR50 |
| I | ILE78 |
| I | PRO79 |
| I | VAL82 |
| I | ALA106 |
| I | SER107 |
| I | PRO129 |
| I | 9TY403 |
| site_id | AF7 |
| Number of Residues | 9 |
| Details | binding site for residue 9TY I 403 |
| Chain | Residue |
| I | SER107 |
| I | ARG130 |
| I | ASP191 |
| I | GLU195 |
| I | MG401 |
| I | NDP402 |
| I | HOH504 |
| J | SER254 |
| J | SER257 |
| site_id | AF8 |
| Number of Residues | 2 |
| Details | binding site for residue MG I 404 |
| Chain | Residue |
| I | GLU227 |
| J | 9TY404 |
| site_id | AF9 |
| Number of Residues | 1 |
| Details | binding site for residue MG J 401 |
| Chain | Residue |
| J | GLU227 |
| site_id | AG1 |
| Number of Residues | 3 |
| Details | binding site for residue MG J 402 |
| Chain | Residue |
| J | ASP191 |
| J | GLU195 |
| J | 9TY404 |
| site_id | AG2 |
| Number of Residues | 12 |
| Details | binding site for residue NDP J 403 |
| Chain | Residue |
| J | TYR23 |
| J | GLY24 |
| J | ASN25 |
| J | GLN26 |
| J | ASN45 |
| J | VAL46 |
| J | ILE78 |
| J | VAL82 |
| J | SER107 |
| J | PRO129 |
| J | VAL132 |
| J | 9TY404 |
| site_id | AG3 |
| Number of Residues | 7 |
| Details | binding site for residue 9TY J 404 |
| Chain | Residue |
| I | SER257 |
| I | MG404 |
| J | ARG130 |
| J | ASP191 |
| J | GLU195 |
| J | MG402 |
| J | NDP403 |
| site_id | AG4 |
| Number of Residues | 3 |
| Details | binding site for residue MG K 401 |
| Chain | Residue |
| K | ASP191 |
| K | GLU195 |
| K | 9TY403 |
| site_id | AG5 |
| Number of Residues | 12 |
| Details | binding site for residue NDP K 402 |
| Chain | Residue |
| K | TYR23 |
| K | GLY24 |
| K | ASN25 |
| K | GLN26 |
| K | ASN45 |
| K | VAL46 |
| K | TYR50 |
| K | ILE78 |
| K | PRO79 |
| K | SER107 |
| K | PRO129 |
| K | 9TY403 |
| site_id | AG6 |
| Number of Residues | 7 |
| Details | binding site for residue 9TY K 403 |
| Chain | Residue |
| K | SER107 |
| K | ASP191 |
| K | GLU195 |
| K | MG401 |
| K | NDP402 |
| L | SER254 |
| L | SER257 |
| site_id | AG7 |
| Number of Residues | 2 |
| Details | binding site for residue MG K 404 |
| Chain | Residue |
| K | GLU227 |
| K | 9TY405 |
| site_id | AG8 |
| Number of Residues | 7 |
| Details | binding site for residue 9TY K 405 |
| Chain | Residue |
| K | SER254 |
| K | SER257 |
| K | MG404 |
| L | ASP191 |
| L | GLU195 |
| L | MG402 |
| L | NDP403 |
| site_id | AG9 |
| Number of Residues | 1 |
| Details | binding site for residue MG L 401 |
| Chain | Residue |
| L | GLU227 |
| site_id | AH1 |
| Number of Residues | 4 |
| Details | binding site for residue MG L 402 |
| Chain | Residue |
| K | SER257 |
| K | 9TY405 |
| L | ASP191 |
| L | GLU195 |
| site_id | AH2 |
| Number of Residues | 12 |
| Details | binding site for residue NDP L 403 |
| Chain | Residue |
| K | 9TY405 |
| L | TYR23 |
| L | GLY24 |
| L | ASN25 |
| L | GLN26 |
| L | ASN45 |
| L | VAL46 |
| L | TYR50 |
| L | ILE78 |
| L | VAL82 |
| L | SER107 |
| L | PRO129 |
| site_id | AH3 |
| Number of Residues | 11 |
| Details | binding site for Di-peptide NDP G 402 and ASN G 25 |
| Chain | Residue |
| G | TYR23 |
| G | GLY24 |
| G | GLN26 |
| G | ASN45 |
| G | VAL46 |
| G | ASP48 |
| G | TYR50 |
| G | VAL82 |
| G | MET131 |
| G | VAL132 |
| G | 9TY403 |
