6JAU

The complex structure of Pseudomonas aeruginosa MucA/MucB.

Summary for 6JAU

• Entry DOI: 10.2210/pdb6jau/pdb
• Descriptor: Sigma factor AlgU regulatory protein MucB, Sigma factor AlgU negative regulatory protein, CALCIUM ION, ... (6 entities in total)
• Functional Keywords: muca/mucb, regulated intramembrane proteolysis, lps, alginate, protein binding
• Biological source: Pseudomonas aeruginosa (strain PAO1); More
• Total number of polymer chains: 2
• Total formula weight: 44709.86

Authors

Li, T.,He, L.H.,Li, C.C.,Liu, L.,Peng, C.T.,Shen, Y.L.,Qin, X.F.,Xiao, Q.J.,Zhu, Y.B.,Song, Y.J.,Zhao, N.l.,Zhao, C.,Yang, J.,Mu, X.Y.,Huang, Q.,Bao, R. (deposition date: 2019-01-25, release date: 2020-01-29, Last modification date: 2024-10-23)

Primary citation

Li, T.,He, L.,Li, C.,Kang, M.,Song, Y.,Zhu, Y.,Shen, Y.,Zhao, N.,Zhao, C.,Yang, J.,Huang, Q.,Mou, X.,Tong, A.,Yang, J.,Wang, Z.,Ji, C.,Li, H.,Tang, H.,Bao, R.
Molecular basis of the lipid-induced MucA-MucB dissociation in Pseudomonas aeruginosa.
Commun Biol, 3:418-418, 2020

PubMed Abstract: MucA and MucB are critical negative modulators of sigma factor AlgU and regulate the mucoid conversion of Pseudomonas aeruginosa. Previous studies have revealed that lipid signals antagonize MucA-MucB binding. Here we report the crystal structure of MucB in complex with the periplasmic domain of MucA and polyethylene glycol (PEG), which unveiled an intermediate state preceding the MucA-MucB dissociation. Based on the biochemical experiments, the aliphatic side chain with a polar group was found to be of primary importance for inducing MucA cleavage. These results provide evidence that the hydrophobic cavity of MucB is a primary site for sensing lipid molecules and illustrates the detailed control of conformational switching within MucA-MucB in response to lipophilic effectors.

PubMed: 32747658
DOI: 10.1038/s42003-020-01147-1

Experimental method

X-RAY DIFFRACTION (1.905 Å)