6JAU

The complex structure of Pseudomonas aeruginosa MucA/MucB.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9776
Spacegroup name	C 2 2 21
Unit cell lengths	71.907, 186.429, 50.628
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.953 - 1.905
R-factor	0.1744
Rwork	0.173
R-free	0.19290
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.028
RMSD bond angle	1.919
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.930
High resolution limit [Å]	1.900	1.900
Number of reflections	26846
<I/σ(I)>	15.57
Completeness [%]	99.9	99.8
Redundancy	10

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	30%PEG550, 0.1M BIS-TRIS PH 6.5, 0.05M CaCl2