6J52

Crystal structure of CARD-only protein in frog virus 3

Summary for 6J52

• Entry DOI: 10.2210/pdb6j52/pdb
• Descriptor: Caspase recruitment domain-only protein (2 entities in total)
• Functional Keywords: apoptosis, death domain superfamily, caspase recruitment domain, card-only protein, domain swapping
• Biological source: Frog virus 3 (FV-3)
• Total number of polymer chains: 4
• Total formula weight: 46273.36

Authors

Park, H.H.,Kwon, S. (deposition date: 2019-01-10, release date: 2019-02-20, Last modification date: 2023-11-22)

Primary citation

Kim, C.M.,Ha, H.J.,Kwon, S.,Jeong, J.H.,Lee, S.H.,Kim, Y.G.,Lee, C.S.,Lee, J.H.,Park, H.H.
Structural transformation-mediated dimerization of caspase recruitment domain revealed by the crystal structure of CARD-only protein in frog virus 3.
J. Struct. Biol., 205:189-195, 2019

PubMed Abstract: Caspase recruitment domain (CARD)-only proteins (COPs), regulate apoptosis, inflammation, and innate immunity. They inhibit the assembly of NOD-like receptor complexes such as the inflammasome and NODosome, which are molecular complexes critical for caspase-1 activation. COPs are known to interact with either caspase-1 CARD or RIP2 CARD via a CARD-CARD interaction, and inhibit caspase-1 activation or further downstream signaling. In addition to the human COPs, Pseudo-ICE, INCA, and ICEBERG, several viruses also contain viral COPs that help them escape the host immune system. To elucidate the molecular mechanism of host immunity inhibition by viral COPs, we solved the structure of a viral COP for the first time. Our structure showed that viral COP forms a structural transformation-mediated dimer, which is unique and has not been reported in any structural study of a CARD domain. Based on the current structure, and the previously solved structures of other death domain superfamily members, we propose that structural transformation-mediated dimerization might be a new strategy for dimer assembly in the death domain superfamily.

PubMed: 30625366
DOI: 10.1016/j.jsb.2018.12.006

Experimental method

X-RAY DIFFRACTION (2.504 Å)