6J52

Crystal structure of CARD-only protein in frog virus 3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	95
Detector technology	CCD
Collection date	2015-11-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9796
Spacegroup name	P 1 21 1
Unit cell lengths	46.657, 60.500, 78.836
Unit cell angles	90.00, 89.82, 90.00

Refinement procedure

Resolution	40.098 - 2.504
R-factor	0.1748
Rwork	0.168
R-free	0.23630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dgn
RMSD bond length	0.007
RMSD bond angle	0.849
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((dev_3139: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.540
High resolution limit [Å]	2.500	2.500
Number of reflections	15193	780
<I/σ(I)>	38.02
Completeness [%]	99.8
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	2.5M NaCl, 0.1M imidazole pH 8.0, 10%(w/v) PEG 3350