6ITE
Crystal structure of group A Streptococcal surface dehydrogenase (SDH)
Summary for 6ITE
| Entry DOI | 10.2210/pdb6ite/pdb |
| Descriptor | Glyceraldehyde-3-phosphate dehydrogenase, NICOTINAMIDE-ADENINE-DINUCLEOTIDE, SULFATE ION, ... (4 entities in total) |
| Functional Keywords | dehydrogenase, glycolysis, streptococcus pyogenes, oxidoreductase |
| Biological source | Streptococcus pyogenes |
| Total number of polymer chains | 4 |
| Total formula weight | 150540.14 |
| Authors | Yuan, C.,Li, R.,Huang, M.D. (deposition date: 2018-11-21, release date: 2019-09-25, Last modification date: 2023-11-22) |
| Primary citation | Li, R.,Liang, C.,Jiang, L.,Yuan, C.,Huang, M. Structural determination of group A Streptococcal surface dehydrogenase and characterization of its interaction with urokinase-type plasminogen activator receptor. Biochem.Biophys.Res.Commun., 510:539-544, 2019 Cited by PubMed Abstract: Streptococcus pyogenes (group A Streptococcus, GAS) has caused a wide variety of human diseases. Its multifunctional surface dehydrogenase (SDH) is crucial for GAS life cycle. Furthermore, GAS infection into human pharyngeal cells has been previously shown to be mediated by the interaction between SDH and host urokinase-type plasminogen activator receptor (uPAR). However, the structural information of SDH remains to be elucidated and there are few detailed studies to characterize its interaction with uPAR. In-depth research on these issues will provide potential targets and strategies for combating GAS. Here, we prepared recombinant SDH tetramer in Escherichia coli BL21 (DE3) cells. After purification and crystallization, we determined its crystal structure at 1.74 Å. The unique characteristics might be potentially explored as drug targets or vaccine immunogen. We subsequently performed gel filtration chromatography, native-polyacrylamide gel electrophoresis (PAGE) and in vitro pull-down analyses. The results showed that their interaction was too weak to form stable complexes and the role of uPAR involved in GAS infection needs further demonstration. Altogether the current work provides the first view of SDH and deepens the knowledge of GAS infection. PubMed: 30737033DOI: 10.1016/j.bbrc.2019.01.102 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.739 Å) |
Structure validation
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