6IOL

Cryo-EM structure of multidrug efflux pump MexAB-OprM (60 degree state)

6IOL の概要

• エントリーDOI: 10.2210/pdb6iol/pdb
• EMDBエントリー: 9696
• 分子名称: Multidrug resistance protein MexA, Outer membrane protein OprM, Multidrug resistance protein MexB (3 entities in total)
• 機能のキーワード: multidrug resistance, efflux, complex, membrane protein
• 由来する生物種: Pseudomonas aeruginosa; 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 729795.80

構造登録者

Tsutsumi, K.,Yonehara, R.,Nakagawa, A.,Yamashita, E. (登録日: 2018-10-30, 公開日: 2019-04-03, 最終更新日: 2025-06-25)

主引用文献

Tsutsumi, K.,Yonehara, R.,Ishizaka-Ikeda, E.,Miyazaki, N.,Maeda, S.,Iwasaki, K.,Nakagawa, A.,Yamashita, E.
Structures of the wild-type MexAB-OprM tripartite pump reveal its complex formation and drug efflux mechanism.
Nat Commun, 10:1520-1520, 2019

PubMed Abstract: In Pseudomonas aeruginosa, MexAB-OprM plays a central role in multidrug resistance by ejecting various drug compounds, which is one of the causes of serious nosocomial infections. Although the structures of the components of MexAB-OprM have been solved individually by X-ray crystallography, no structural information for fully assembled pumps from P. aeruginosa were previously available. In this study, we present the structure of wild-type MexAB-OprM in the presence or absence of drugs at near-atomic resolution. The structure reveals that OprM does not interact with MexB directly, and that it opens its periplasmic gate by forming a complex. Furthermore, we confirm the residues essential for complex formation and observed a movement of the drug entrance gate. Based on these results, we propose mechanisms for complex formation and drug efflux.

PubMed: 30944318
DOI: 10.1038/s41467-019-09463-9

実験手法

ELECTRON MICROSCOPY (3.76 Å)