6I4B

Plasmodium falciparum dihydroorotate dehydrogenase (DHODH) co-crystallized with 3-Hydroxy-1-methyl-5-((3-(trifluoromethyl)phenoxy)methyl)-1H-pyrazole-4-carboxylic acid

Summary for 6I4B

• Entry DOI: 10.2210/pdb6i4b/pdb
• Descriptor: Dihydroorotate dehydrogenase, FLAVIN MONONUCLEOTIDE, OROTIC ACID, ... (5 entities in total)
• Functional Keywords: dhodh, plasmodium falciparum dhodh, oxidoreductase
• Biological source: Plasmodium falciparum (malaria parasite P. falciparum)
• Total number of polymer chains: 2
• Total formula weight: 93562.27

Authors

Goyal, P.,Sainas, S.,Pippione, A.C.,Boschi, D.,Al-Kadaraghi, S. (deposition date: 2018-11-09, release date: 2018-12-19, Last modification date: 2024-05-15)

Primary citation

Pippione, A.C.,Sainas, S.,Goyal, P.,Fritzson, I.,Cassiano, G.C.,Giraudo, A.,Giorgis, M.,Tavella, T.A.,Bagnati, R.,Rolando, B.,Caing-Carlsson, R.,Costa, F.T.M.,Andrade, C.H.,Al-Karadaghi, S.,Boschi, D.,Friemann, R.,Lolli, M.L.
Hydroxyazole scaffold-based Plasmodium falciparum dihydroorotate dehydrogenase inhibitors: Synthesis, biological evaluation and X-ray structural studies.
Eur J Med Chem, 163:266-280, 2018

PubMed Abstract: Plasmodium falciparum dihydroorotate dehydrogenase (PfDHODH) has been clinically validated as a target for antimalarial drug discovery, as a triazolopyrimidine class inhibitor (DSM265) is currently undergoing clinical development. Here, we have identified new hydroxyazole scaffold-based PfDHODH inhibitors belonging to two different chemical series. The first series was designed by a scaffold hopping strategy that exploits the use of hydroxylated azoles. Within this series, the hydroxythiadiazole 3 was identified as the best selective PfDHODH inhibitor (IC 12.0 μM). The second series was designed by modulating four different positions of the hydroxypyrazole scaffold. In particular, hydroxypyrazoles 7e and 7f were shown to be active in the low μM range (IC 2.8 and 5.3 μM, respectively). All three compounds, 3, 7e and 7f showed clear selectivity over human DHODH (IC > 200 μM), low cytotoxicity, and retained micromolar activity in P. falciparum-infected erythrocytes. The crystallographic structures of PfDHODH in complex with compounds 3 and 7e proved their binding mode, supplying essential data for future optimization of these scaffolds.

PubMed: 30529545
DOI: 10.1016/j.ejmech.2018.11.044

Experimental method

X-RAY DIFFRACTION (1.98 Å)