6I00

Cryo-EM informed directed evolution of Nitrilase 4 leads to a change in quaternary structure.

6I00 の概要

• エントリーDOI: 10.2210/pdb6i00/pdb
• EMDBエントリー: 0320
• 分子名称: Bifunctional nitrilase/nitrile hydratase NIT4 (1 entity in total)
• 機能のキーワード: nitrilase, cyanide detoxification, beta-cyano-l-alanine hydrolase, helical filament, hydrolase
• 由来する生物種: Arabidopsis thaliana (Mouse-ear cress)
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 477193.36

構造登録者

Mulelu, A.E.,Woodward, J.D. (登録日: 2018-10-24, 公開日: 2019-07-24, 最終更新日: 2024-05-15)

• 主引用文献: Mulelu, A.E.,Kirykowicz, A.M.,Woodward, J.D.; Cryo-EM and directed evolution reveal howArabidopsisnitrilase specificity is influenced by its quaternary structure.; Commun Biol, 2:260-260, 2019; PubMed: 31341959; DOI: 10.1038/s42003-019-0505-4

実験手法

ELECTRON MICROSCOPY (3.4 Å)