6HU9

III2-IV2 mitochondrial respiratory supercomplex from S. cerevisiae

Summary for 6HU9

• Entry DOI: 10.2210/pdb6hu9/pdb
• EMDB information: 0262 0268 0269
• Descriptor: Cytochrome b-c1 complex subunit 1, mitochondrial, Cytochrome b-c1 complex subunit 10, Cytochrome c oxidase subunit 1, ... (35 entities in total)
• Functional Keywords: cytochrome c oxidase cytochrome bc1 mitochondria respiratory chain supercomplex, oxidoreductase, electron transport, oxidoreductase-electron transport complex, oxidoreductase/electron transport
• Biological source: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast); More
• Total number of polymer chains: 44
• Total formula weight: 936427.10

Authors

Hartley, A.M.,Pinotsis, N.,Marechal, A. (deposition date: 2018-10-05, release date: 2018-12-26, Last modification date: 2024-11-20)

Primary citation

Hartley, A.M.,Lukoyanova, N.,Zhang, Y.,Cabrera-Orefice, A.,Arnold, S.,Meunier, B.,Pinotsis, N.,Marechal, A.
Structure of yeast cytochrome c oxidase in a supercomplex with cytochrome bc1.
Nat. Struct. Mol. Biol., 26:78-83, 2019

PubMed Abstract: Cytochrome c oxidase (complex IV, CIV) is known in mammals to exist independently or in association with other respiratory proteins to form supercomplexes (SCs). In Saccharomyces cerevisiae, CIV is found solely in an SC with cytochrome bc (complex III, CIII). Here, we present the cryogenic electron microscopy (cryo-EM) structure of S. cerevisiae CIV in a IIIIV SC at 3.3 Å resolution. While overall similarity to mammalian homologs is high, we found notable differences in the supernumerary subunits Cox26 and Cox13; the latter exhibits a unique arrangement that precludes CIV dimerization as seen in bovine. A conformational shift in the matrix domain of Cox5A-involved in allosteric inhibition by ATP-may arise from its association with CIII. The CIII-CIV arrangement highlights a conserved interaction interface of CIII, albeit one occupied by complex I in mammalian respirasomes. We discuss our findings in the context of the potential impact of SC formation on CIV regulation.

PubMed: 30598554
DOI: 10.1038/s41594-018-0172-z

Experimental method

ELECTRON MICROSCOPY (3.35 Å)