6HU9

III2-IV2 mitochondrial respiratory supercomplex from S. cerevisiae

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, K (A, L)	Cytochrome b-c1 complex subunit 1, mitochondrial	polymer	431	47459.3	2	UniProt (P07256) Pfam (PF00675) Pfam (PF05193)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Complex III subunit 1,Core protein I,Ubiquinol-cytochrome-c reductase complex core protein 1
2	B, L (B, M)	Cytochrome b-c1 complex subunit 2, mitochondrial	polymer	352	38751.9	2	UniProt (P07257) Pfam (PF00675)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Complex III subunit 2,Core protein II,Ubiquinol-cytochrome-c reductase complex core protein 2
3	C, M (C, N)	Cytochrome b	polymer	385	43686.6	2	UniProt (P00163) Pfam (PF00033) Pfam (PF00032)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Complex III subunit 3,Complex III subunit CYTB,Complex III subunit III,Cytochrome b-c1 complex subunit 3,Cytochrome b-c1 complex subunit CYTB,Ubiquinol-cytochrome-c reductase complex cytochrome b subunit
4	D, N (D, O)	Cytochrome c1, heme protein, mitochondrial	polymer	248	27807.4	2	UniProt (P07143) Pfam (PF02167)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Complex III subunit 4,Complex III subunit IV,Cytochrome b-c1 complex subunit 4,Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit,Cytochrome c-1
5	E, O (E, P)	Cytochrome b-c1 complex subunit Rieske, mitochondrial	polymer	185	20123.0	2	UniProt (P08067) Pfam (PF02921) Pfam (PF00355)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Complex III subunit 5,Rieske iron-sulfur protein,RISP,Ubiquinol-cytochrome c reductase iron-sulfur subunit
6	F, P (F, Q)	Cytochrome b-c1 complex subunit 6	polymer	147	17276.1	2	UniProt (P00127) Pfam (PF02320)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Complex III subunit 6,Complex III subunit VI,Cytochrome c1 non-heme 17 kDa protein,Mitochondrial hinge protein,Ubiquinol-cytochrome c reductase complex 17 kDa protein
7	G, Q (G, R)	Cytochrome b-c1 complex subunit 7	polymer	127	14583.8	2	UniProt (P00128) Pfam (PF02271)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Complex III subunit 7,Complex III subunit VII,Ubiquinol-cytochrome c reductase c reductase complex 14 kDa protein
8	H, R (H, S)	Cytochrome b-c1 complex subunit 8	polymer	93	10856.3	2	UniProt (P08525) Pfam (PF02939)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Complex III subunit 8,Complex III subunit VII,Ubiquinol-cytochrome c reductase complex 11 kDa protein,Ubiquinol-cytochrome c reductase complex ubiquinone-binding protein QP-C
9	I, S (I, T)	Cytochrome b-c1 complex subunit 9	polymer	66	7485.3	2	UniProt (P22289) Pfam (PF05365)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Complex III subunit 9,Complex III subunit X,Cytochrome c1 non-heme 7.3 kDa protein,Ubiquinol-cytochrome c reductase complex 7.3 kDa protein
10	J, T (J, U)	Cytochrome b-c1 complex subunit 10	polymer	77	8602.9	2	UniProt (P37299) Pfam (PF09796)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Complex III subunit 10,Complex III subunit XI,Ubiquinol-cytochrome c reductase complex 8.5 kDa protein
11	GA, U (a, m)	Cytochrome c oxidase subunit 1	polymer	534	58832.6	2	UniProt (P00401) Pfam (PF00115)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Cytochrome c oxidase polypeptide I, COX1
12	HA, V (b, n)	Cytochrome c oxidase subunit 2	polymer	236	26779.8	2	UniProt (P00410) Pfam (PF02790) Pfam (PF00116)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Cytochrome c oxidase polypeptide II, COX2
13	IA, W (c, o)	Cytochrome c oxidase subunit 3	polymer	269	30383.6	2	UniProt (P00420) Pfam (PF00510)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Cytochrome c oxidase polypeptide III, COX3
14	JA, X (d, p)	Cytochrome c oxidase subunit 4, mitochondrial	polymer	130	14188.9	2	UniProt (P04037) Pfam (PF01215)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Cytochrome c oxidase polypeptide IV, COX4
15	KA, Y (e, q)	Cytochrome c oxidase polypeptide 5A, mitochondrial	polymer	133	14891.8	2	UniProt (P00424) Pfam (PF02936)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Cytochrome c oxidase polypeptide Va, COX5A
16	LA, Z (f, r)	Cytochrome c oxidase subunit 6, mitochondrial	polymer	108	12642.0	2	UniProt (P00427) Pfam (PF02284)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Cytochrome c oxidase polypeptide VI, COX6
17	AA, MA (g, s)	Cytochrome c oxidase subunit 7	polymer	59	6811.2	2	UniProt (P10174) Pfam (PF02238)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Cytochrome c oxidase polypeptide VII, COX7
18	BA, NA (h, t)	Cytochrome c oxidase polypeptide VIII, mitochondrial	polymer	47	5375.4	2	UniProt (P04039) Pfam (PF02935)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
19	CA, OA (i, u)	Cytochrome c oxidase subunit 7A	polymer	55	6471.7	2	UniProt (P07255)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Cytochrome c oxidase polypeptide VIIA, COX9
20	DA, PA (j, v)	Cytochrome c oxidase subunit 6B	polymer	82	9668.7	2	UniProt (Q01519) Pfam (PF02297)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Cytochrome c oxidase polypeptide VIb, COX12
21	EA, QA (k, w)	Cytochrome c oxidase subunit 6A, mitochondrial	polymer	131	15117.0	2	UniProt (P32799) Pfam (PF02046)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Cytochrome c oxidase polypeptide VIa, COX13
22	FA, RA (l, x)	Cox26	polymer	66	7461.7	2	UniProt (Q2V2P9)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
23	BB, BD, CD, DD, EB... (D, n, o, E, a...)	DI-PALMITOYL-3-SN-PHOSPHATIDYLETHANOLAMINE	non-polymer		692.0	28	Chemie (PEF)
24	LB, MB, TA, UA (N, C)	PROTOPORPHYRIN IX CONTAINING FE	non-polymer		616.5	4	Chemie (HEM)
25	VA (C)	5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL	non-polymer		592.9	1	Chemie (UQ6)
26	DB, FB, GB, KB, SB... (E, H, L, O, P...)	CARDIOLIPIN	non-polymer		1464.0	8	Chemie (CDL)
27	AC, HD, IB, JB, QB... (T, q, H, I, N...)	1,2-DIACYL-SN-GLYCERO-3-PHOSHOCHOLINE	non-polymer		734.0	8	Chemie (PCF)
28	AB, RB (D, O)	HEME C	non-polymer		618.5	2	Chemie (HEC)
29	CB, UB (E, P)	FE2/S2 (INORGANIC) CLUSTER	non-polymer		175.8	2	Chemie (FES)
30	BC, SC (a, m)	COPPER (II) ION	non-polymer		63.5	2	Chemie (CU)
31	CC, DC, TC, UC (a, m)	HEME-A	non-polymer		852.8	4	Chemie (HEA)
32	EC, VC (a, m)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
33	FC, WC (a, m)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
34	AD, JC, KC, ZC (n, b)	DINUCLEAR COPPER ION	non-polymer		127.1	4	Chemie (CUA)
35	FD, OC (p, d)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)

Sequence modifications

k, w: 10 - 129 (UniProt: P32799)

PDB	External Database	Details
Gly 130	-	expression tag
Ala 131	-	expression tag
Arg 132	-	expression tag
Gly 133	-	expression tag
Ser 134	-	expression tag
His 135	-	expression tag
His 136	-	expression tag
His 137	-	expression tag
His 138	-	expression tag
His 139	-	expression tag
His 140	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	44
	Total formula weight	890513.7
Non-Polymers*	Number of molecules	69
	Total formula weight	45913.4
All*	Total formula weight	936427.1

*Water molecules are not included.