6HU5

STRUCTURE OF HEWL BY ELECTRON DIFFRACTION AND MICROFOCUS DIFFRACTION

6HU5 の概要

• エントリーDOI: 10.2210/pdb6hu5/pdb
• 関連するPDBエントリー: 1LCN 1LKR 3J6K 5A3E 5K7O 5O4W 5OCV 6HT2
• 分子名称: Lysozyme C, CHLORIDE ION (2 entities in total)
• 機能のキーワード: lysozyme, hewl, ed, electron, diffraction, hydrolase, crystal, chloride, halogen, protein, dimer, microfocus
• 由来する生物種: Gallus gallus (Chicken)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 28768.68

構造登録者

Garau, G. (登録日: 2018-10-05, 公開日: 2019-01-23, 最終更新日: 2024-11-13)

主引用文献

Lanza, A.,Margheritis, E.,Mugnaioli, E.,Cappello, V.,Garau, G.,Gemmi, M.
Nanobeam precession-assisted 3D electron diffraction reveals a new polymorph of hen egg-white lysozyme.
Iucrj, 6:178-188, 2019

PubMed Abstract: Recent advances in 3D electron diffraction have allowed the structure determination of several model proteins from submicrometric crystals, the unit-cell parameters and structures of which could be immediately validated by known models previously obtained by X-ray crystallography. Here, the first new protein structure determined by 3D electron diffraction data is presented: a previously unobserved polymorph of hen egg-white lysozyme. This form, with unit-cell parameters = 31.9, = 54.4, = 71.8 Å, β = 98.8°, grows as needle-shaped submicrometric crystals simply by vapor diffusion starting from previously reported crystallization conditions. Remarkably, the data were collected using a low-dose stepwise experimental setup consisting of a precession-assisted nanobeam of ∼150 nm, which has never previously been applied for solving protein structures. The crystal structure was additionally validated using X-ray synchrotron-radiation sources by both powder diffraction and single-crystal micro-diffraction. 3D electron diffraction can be used for the structural characterization of submicrometric macromolecular crystals and is able to identify novel protein polymorphs that are hardly visible in conventional X-ray diffraction experiments. Additionally, the analysis, which was performed on both nanocrystals and microcrystals from the same crystallization drop, suggests that an integrated view from 3D electron diffraction and X-ray microfocus diffraction can be applied to obtain insights into the molecular dynamics during protein crystal growth.

PubMed: 30867915
DOI: 10.1107/S2052252518017657

実験手法

ELECTRON CRYSTALLOGRAPHY (2.8 Å)