5OCV

A Rare Lysozyme Crystal Form Solved Using High-Redundancy 3D Electron Diffraction Data from Micron-Sized Needle Shaped Crystals

Summary for 5OCV

• Entry DOI: 10.2210/pdb5ocv/pdb
• Descriptor: Lysozyme C, SODIUM ION (3 entities in total)
• Functional Keywords: lysozyme activity, hydrolase
• Biological source: Gallus gallus (Chicken)
• Cellular location: Secreted: P00698
• Total number of polymer chains: 2
• Total formula weight: 28685.31

Authors

Xu, H.,Lebrette, H.,Yang, T.,Srinivas, V.,Hovmoller, S.,Hogbom, M.,Zou, X. (deposition date: 2017-07-03, release date: 2018-03-28, Last modification date: 2024-11-13)

Primary citation

Xu, H.,Lebrette, H.,Yang, T.,Srinivas, V.,Hovmoller, S.,Hogbom, M.,Zou, X.
A Rare Lysozyme Crystal Form Solved Using Highly Redundant Multiple Electron Diffraction Datasets from Micron-Sized Crystals.
Structure, 26:667-675.e3, 2018

PubMed Abstract: Recent developments of novel electron diffraction techniques have shown to be powerful for determination of atomic resolution structures from micron- and nano-sized crystals, too small to be studied by single-crystal X-ray diffraction. In this work, the structure of a rare lysozyme polymorph is solved and refined using continuous rotation MicroED data and standard X-ray crystallographic software. Data collection was performed on a standard 200 kV transmission electron microscope (TEM) using a highly sensitive detector with a short readout time. The data collection is fast (∼3 min per crystal), allowing multiple datasets to be rapidly collected from a large number of crystals. We show that merging data from 33 crystals significantly improves not only the data completeness, overall I/σ and the data redundancy, but also the quality of the final atomic model. This is extremely useful for electron beam-sensitive crystals of low symmetry or with a preferred orientation on the TEM grid.

PubMed: 29551291
DOI: 10.1016/j.str.2018.02.015

Experimental method

ELECTRON CRYSTALLOGRAPHY (2.2 Å)