6HQV
Pentafunctional AROM Complex from Chaetomium thermophilum
Summary for 6HQV
| Entry DOI | 10.2210/pdb6hqv/pdb |
| Descriptor | Pentafunctional AROM polypeptide, NICOTINAMIDE-ADENINE-DINUCLEOTIDE, ZINC ION, ... (7 entities in total) |
| Functional Keywords | multifunctional enzyme, shikimate pathway, aromatic amino acid biosynthesis, 3-dehydroquinate synthase, 3-dehydroquinate dehydratase, shikimate dehydrogenase, shikimate kinase, 3-phosphoshikimate 1-carboxyvinyltransferase, 5-enolpyruvylshikimate-3-phosphate synthase, biosynthetic protein |
| Biological source | Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) |
| Total number of polymer chains | 2 |
| Total formula weight | 352942.15 |
| Authors | Arora Verasto, H.,Hartmann, M.D. (deposition date: 2018-09-25, release date: 2020-05-06, Last modification date: 2024-11-13) |
| Primary citation | Arora Veraszto, H.,Logotheti, M.,Albrecht, R.,Leitner, A.,Zhu, H.,Hartmann, M.D. Architecture and functional dynamics of the pentafunctional AROM complex. Nat.Chem.Biol., 16:973-978, 2020 Cited by PubMed Abstract: The AROM complex is a multifunctional metabolic machine with ten enzymatic domains catalyzing the five central steps of the shikimate pathway in fungi and protists. We determined its crystal structure and catalytic behavior, and elucidated its conformational space using a combination of experimental and computational approaches. We derived this space in an elementary approach, exploiting an abundance of conformational information from its monofunctional homologs in the Protein Data Bank. It demonstrates how AROM is optimized for spatial compactness while allowing for unrestricted conformational transitions and a decoupled functioning of its individual enzymatic entities. With this architecture, AROM poses a tractable test case for the effects of active site proximity on the efficiency of both natural metabolic systems and biotechnological pathway optimization approaches. We show that a mere colocalization of enzymes is not sufficient to yield a detectable improvement of metabolic throughput. PubMed: 32632294DOI: 10.1038/s41589-020-0587-9 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3 Å) |
Structure validation
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