6HIK

X-ray structure of TEAD4(Y429H) mutant) complexed with YAP (wildtype): Molecular and structural characterization of a TEAD mutation at the origin of Sveinsson's chorioretinal atrophy

Summary for 6HIK

• Entry DOI: 10.2210/pdb6hik/pdb
• Related: 6GE3
• Descriptor: Transcriptional enhancer factor TEF-3, Transcriptional coactivator YAP1, MYRISTIC ACID, ... (5 entities in total)
• Functional Keywords: transcription
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 2
• Total formula weight: 30441.24

Authors

Kallen, J. (deposition date: 2018-08-30, release date: 2019-04-03, Last modification date: 2024-11-06)

Primary citation

Bokhovchuk, F.,Mesrouze, Y.,Izaac, A.,Meyerhofer, M.,Zimmermann, C.,Fontana, P.,Schmelzle, T.,Erdmann, D.,Furet, P.,Kallen, J.,Chene, P.
Molecular and structural characterization of a TEAD mutation at the origin of Sveinsson's chorioretinal atrophy.
Febs J., 286:2381-2398, 2019

PubMed Abstract: Four TEAD transcription factors (TEAD1-4) mediate the signalling output of the Hippo pathway that controls organ size in humans. TEAD transcriptional activity is regulated via interactions with the YAP, TAZ and VGLL proteins. A mutation in the TEAD1 gene, Tyr421His, has been identified in patients suffering from Sveinsson's chorioretinal atrophy (SCA), an autosomal dominant eye disease. This mutation prevents the YAP/TAZ:TEAD1 interaction. In this study, we measure the affinity of YAP, TAZ and VGLL1 for the four human TEADs and find that they have a similar affinity for all TEADs. We quantitate the effect of the mutation found in SCA patients and show that it destabilizes the YAP/TAZ:TEAD interaction by about 3 kcal·mol . We determine the structure of YAP in complex with this mutant form of TEAD and show that the histidine residue adopts different conformations at the binding interface. The presence of this flexible residue induces the destabilization of several H-bonds and the loss of van der Waals contacts, which explains why the Tyr421His mutation has such a large destabilizing effect on the formation of the YAP:TEAD complex. DATABASE: The crystallographic data have been deposited at the RSCB Protein Data Bank (PDB, www.pdb.org) with the access codes: 6HIL (wt :Tyr421His ), 6HIK (wt :Tyr429His ).

PubMed: 30903741
DOI: 10.1111/febs.14817

Experimental method

X-RAY DIFFRACTION (1.65 Å)