6H82
Cryo-EM structure of the archaeal extremophilic internal membrane containing Haloarcula hispanica icosahedral virus 2 (HHIV-2) at 3.78 Angstroms resolution.
Summary for 6H82
| Entry DOI | 10.2210/pdb6h82/pdb |
| EMDB information | 0050 0072 0073 0131 0172 |
| Descriptor | VP4, VP7, Uncharacterized protein, ... (8 entities in total) |
| Functional Keywords | single vertical beta-barrel virus, archaeal, membrane-containing, quasi-atomic resolution, virus |
| Biological source | Haloarcula hispanica icosahedral virus 2 More |
| Total number of polymer chains | 32 |
| Total formula weight | 636572.27 |
| Authors | Abrescia, N.G.,Santos-Perez, I.,Charro, D. (deposition date: 2018-08-01, release date: 2019-04-03, Last modification date: 2024-05-15) |
| Primary citation | Santos-Perez, I.,Charro, D.,Gil-Carton, D.,Azkargorta, M.,Elortza, F.,Bamford, D.H.,Oksanen, H.M.,Abrescia, N.G.A. Structural basis for assembly of vertical single beta-barrel viruses. Nat Commun, 10:1184-1184, 2019 Cited by PubMed Abstract: The vertical double β-barrel major capsid protein (MCP) fold, fingerprint of the PRD1-adeno viral lineage, is widespread in many viruses infecting organisms across the three domains of life. The discovery of PRD1-like viruses with two MCPs challenged the known assembly principles. Here, we present the cryo-electron microscopy (cryo-EM) structures of the archaeal, halophilic, internal membrane-containing Haloarcula californiae icosahedral virus 1 (HCIV-1) and Haloarcula hispanica icosahedral virus 2 (HHIV-2) at 3.7 and 3.8 Å resolution, respectively. Our structures reveal proteins located beneath the morphologically distinct two- and three-tower capsomers and homopentameric membrane proteins at the vertices that orchestrate the positioning of pre-formed vertical single β-barrel MCP heterodimers. The cryo-EM based structures together with the proteomics data provide insights into the assembly mechanism of this type of viruses and into those with membrane-less double β-barrel MCPs. PubMed: 30862777DOI: 10.1038/s41467-019-08927-2 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.78 Å) |
Structure validation
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