6H3U

Schmallenberg Virus Glycoprotein Gc Head Domain in Complex with scFv 4B6

Summary for 6H3U

• Entry DOI: 10.2210/pdb6h3u/pdb
• Descriptor: scFv 4B6 VH, scFv 4B6 VL, Envelopment polyprotein, ... (5 entities in total)
• Functional Keywords: envelope glycoprotein, scfv antibody, viral protein
• Biological source: Mus musculus; More
• Total number of polymer chains: 6
• Total formula weight: 116315.48

Authors

Hellert, J.,Aebischer, A.,Wernike, K.,Haouz, A.,Brocchi, E.,Reiche, S.,Guardado-Calvo, P.,Beer, M.,Rey, F.A. (deposition date: 2018-07-19, release date: 2019-02-27, Last modification date: 2024-01-17)

Primary citation

Hellert, J.,Aebischer, A.,Wernike, K.,Haouz, A.,Brocchi, E.,Reiche, S.,Guardado-Calvo, P.,Beer, M.,Rey, F.A.
Orthobunyavirus spike architecture and recognition by neutralizing antibodies.
Nat Commun, 10:879-879, 2019

PubMed Abstract: Orthobunyaviruses (OBVs) form a distinct genus of arthropod-borne bunyaviruses that can cause severe disease upon zoonotic transmission to humans. Antigenic drift or genome segment re-assortment have in the past resulted in new pathogenic OBVs, making them potential candidates for causing emerging zoonoses in the future. Low-resolution electron cryo-tomography studies have shown that OBV particles feature prominent trimeric spikes, but their molecular organization remained unknown. Here we report X-ray crystallography studies of four different OBVs showing that the spikes are formed by an N-terminal extension of the fusion glycoprotein Gc. Using Schmallenberg virus, a recently emerged OBV, we also show that the projecting spike is the major target of the neutralizing antibody response, and provide X-ray structures in complex with two protecting antibodies. We further show that immunization of mice with the spike domains elicits virtually sterilizing immunity, providing fundamental knowledge essential in the preparation for potential newly emerging OBV zoonoses.

PubMed: 30787296
DOI: 10.1038/s41467-019-08832-8

Experimental method

X-RAY DIFFRACTION (3.168 Å)