6GOY
Structure of mEos4b in the green fluorescent state
Summary for 6GOY
| Entry DOI | 10.2210/pdb6goy/pdb |
| Related | 6GOZ 6GP0 6GP1 |
| Descriptor | Green to red photoconvertible GFP-like protein EosFP, DI(HYDROXYETHYL)ETHER, GLYCEROL, ... (5 entities in total) |
| Functional Keywords | fluorescent protein |
| Biological source | Lobophyllia hemprichii (Lobed brain coral) |
| Total number of polymer chains | 1 |
| Total formula weight | 29932.71 |
| Authors | De Zitter, E.,Adam, V.,Byrdin, M.,Van Meervelt, L.,Dedecker, P.,Bourgeois, D. (deposition date: 2018-06-04, release date: 2019-05-22, Last modification date: 2024-10-23) |
| Primary citation | De Zitter, E.,Thedie, D.,Monkemoller, V.,Hugelier, S.,Beaudouin, J.,Adam, V.,Byrdin, M.,Van Meervelt, L.,Dedecker, P.,Bourgeois, D. Mechanistic investigation of mEos4b reveals a strategy to reduce track interruptions in sptPALM. Nat.Methods, 16:707-710, 2019 Cited by PubMed Abstract: Green-to-red photoconvertible fluorescent proteins repeatedly enter dark states, causing interrupted tracks in single-particle-tracking localization microscopy (sptPALM). We identified a long-lived dark state in photoconverted mEos4b that results from isomerization of the chromophore and efficiently absorbs cyan light. Addition of weak 488-nm light swiftly reverts this dark state to the fluorescent state. This strategy largely eliminates slow blinking and enables the recording of longer tracks in sptPALM with minimum effort. PubMed: 31285624DOI: 10.1038/s41592-019-0462-3 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.65 Å) |
Structure validation
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