6GP1

Structure of mEos4b in the red long-lived dark state

Summary for 6GP1

• Entry DOI: 10.2210/pdb6gp1/pdb
• Related: 6GOY 6GOZ 6GP0
• Descriptor: Green to red photoconvertible GFP-like protein EosFP (3 entities in total)
• Functional Keywords: fluorescent protein
• Biological source: Lobophyllia hemprichii (Lobed brain coral); More
• Total number of polymer chains: 2
• Total formula weight: 29566.30

Authors

De Zitter, E.,Adam, V.,Byrdin, M.,Van Meervelt, L.,Dedecker, P.,Bourgeois, D. (deposition date: 2018-06-04, release date: 2019-05-22, Last modification date: 2024-01-17)

Primary citation

De Zitter, E.,Thedie, D.,Monkemoller, V.,Hugelier, S.,Beaudouin, J.,Adam, V.,Byrdin, M.,Van Meervelt, L.,Dedecker, P.,Bourgeois, D.
Mechanistic investigation of mEos4b reveals a strategy to reduce track interruptions in sptPALM.
Nat.Methods, 16:707-710, 2019

PubMed Abstract: Green-to-red photoconvertible fluorescent proteins repeatedly enter dark states, causing interrupted tracks in single-particle-tracking localization microscopy (sptPALM). We identified a long-lived dark state in photoconverted mEos4b that results from isomerization of the chromophore and efficiently absorbs cyan light. Addition of weak 488-nm light swiftly reverts this dark state to the fluorescent state. This strategy largely eliminates slow blinking and enables the recording of longer tracks in sptPALM with minimum effort.

PubMed: 31285624
DOI: 10.1038/s41592-019-0462-3

Experimental method

X-RAY DIFFRACTION (1.504 Å)