6GP1

Structure of mEos4b in the red long-lived dark state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-27
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	38.700, 58.290, 104.030
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.241 - 1.504
R-factor	0.2516
Rwork	0.249
R-free	0.30430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6gp0
RMSD bond length	0.007
RMSD bond angle	0.941
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.11)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	32.241	32.241	1.330
High resolution limit [Å]	1.300	5.810	1.300
Rmerge	0.047	0.020	0.474
Rmeas	0.054	0.023	0.577
Total number of observations	201864
Number of reflections	56087	672	4069
<I/σ(I)>	16.52	54.06	2.14
Completeness [%]	97.7	90.4	96.8
Redundancy	3.599	3.49	2.753
CC(1/2)	0.999	0.999	0.741

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	100 mM HEPES, 30 % PEG 1000