6G8K

14-3-3sigma in complex with a S131beta3S mutated YAP pS127 phosphopeptide

Summary for 6G8K

Entry DOI	10.2210/pdb6g8k/pdb
Related	6g6x 6g8i 6g8j
Descriptor	14-3-3 protein sigma, ACE-ARG-ALA-HIS-SEP-SER-PRO-ALA-BSE-LEU-GLN, CHLORIDE ION, ... (6 entities in total)
Functional Keywords	beta amino acid, hippo pathway, yap/taz, oncoprotein
Biological source	Homo sapiens (Human) More
Total number of polymer chains	2
Total formula weight	27836.30
Authors	Andrei, S.A.,Thijssen, V.,Brunsveld, L.,Ottmann, C.,Milroy, L.G. (deposition date: 2018-04-09, release date: 2019-04-17, Last modification date: 2024-11-13)
Primary citation	Andrei, S.A.,Thijssen, V.,Brunsveld, L.,Ottmann, C.,Milroy, L.G. A study on the effect of synthetic alpha-to-beta3-amino acid mutations on the binding of phosphopeptides to 14-3-3 proteins. Chem.Commun.(Camb.), 55:14809-14812, 2019 Cited by PubMed Abstract: Here we describe the synthesis of a series of α,β-phosphopeptides, based on the phosphoepitope site on YAP1 (yes-associated protein 1), and the biochemical, biophysical and structural characterization of their binding to 14-3-3 proteins. The impact of systematic mono- and di-substitution of α → β3 amino acid residues around the phosphoserine residue are discussed. Our results confirm the important role played by the +2 proline residue in the thermodynamics and structure of the phosphoepitope/14-3-3 interaction. PubMed: 31763628 DOI: 10.1039/c9cc07982c PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.25 Å)

Structure validation