6G8J

14-3-3sigma in complex with a A130beta3A mutated YAP pS127 phosphopeptide

Summary for 6G8J

• Entry DOI: 10.2210/pdb6g8j/pdb
• Related: 6g6x
• Descriptor: 14-3-3 protein sigma, ACE-ARG-ALA-HIS-SEP-SER-PRO-BAL-SER-LEU-GLN, CHLORIDE ION, ... (6 entities in total)
• Functional Keywords: beta amino acid, hippo pathway, yap/taz, oncoprotein
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 2
• Total formula weight: 27800.85

Authors

Andrei, S.A.,Thijssen, V.,Brunsveld, L.,Ottmann, C.,Milroy, L.G. (deposition date: 2018-04-09, release date: 2019-04-17, Last modification date: 2024-01-31)

Primary citation

Andrei, S.A.,Thijssen, V.,Brunsveld, L.,Ottmann, C.,Milroy, L.G.
A study on the effect of synthetic alpha-to-beta3-amino acid mutations on the binding of phosphopeptides to 14-3-3 proteins.
Chem.Commun.(Camb.), 55:14809-14812, 2019

PubMed Abstract: Here we describe the synthesis of a series of α,β-phosphopeptides, based on the phosphoepitope site on YAP1 (yes-associated protein 1), and the biochemical, biophysical and structural characterization of their binding to 14-3-3 proteins. The impact of systematic mono- and di-substitution of α → β3 amino acid residues around the phosphoserine residue are discussed. Our results confirm the important role played by the +2 proline residue in the thermodynamics and structure of the phosphoepitope/14-3-3 interaction.

PubMed: 31763628
DOI: 10.1039/c9cc07982c

Experimental method

X-RAY DIFFRACTION (1.47 Å)