6FUW

Cryo-EM structure of the human CPSF160-WDR33-CPSF30 complex bound to the PAS AAUAAA motif at 3.1 Angstrom resolution

Replaces:  6FBS

Summary for 6FUW

• Entry DOI: 10.2210/pdb6fuw/pdb
• EMDB information: 4225 4225
• Descriptor: Cleavage and polyadenylation specificity factor subunit 1, pre-mRNA 3' end processing protein WDR33, Cleavage and polyadenylation specificity factor subunit 4, ... (5 entities in total)
• Functional Keywords: 3' pre-mrna processing, polyadenylation, cpsf, beta propeller, aauaaa, rna binding protein
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 4
• Total formula weight: 232646.51

Authors

Clerici, M.,Faini, M.,Jinek, M. (deposition date: 2018-02-28, release date: 2018-03-21, Last modification date: 2024-07-10)

Primary citation

Clerici, M.,Faini, M.,Muckenfuss, L.M.,Aebersold, R.,Jinek, M.
Structural basis of AAUAAA polyadenylation signal recognition by the human CPSF complex.
Nat. Struct. Mol. Biol., 25:135-138, 2018

PubMed Abstract: Mammalian mRNA biogenesis requires specific recognition of a hexanucleotide AAUAAA motif in the polyadenylation signals (PAS) of precursor mRNA (pre-mRNA) transcripts by the cleavage and polyadenylation specificity factor (CPSF) complex. Here we present a 3.1-Å-resolution cryo-EM structure of a core CPSF module bound to the PAS hexamer motif. The structure reveals the molecular interactions responsible for base-specific recognition, providing a rationale for mechanistic differences between mammalian and yeast 3' polyadenylation.

PubMed: 29358758
DOI: 10.1038/s41594-017-0020-6

Experimental method

ELECTRON MICROSCOPY (3.07 Å)