6FUW

Cryo-EM structure of the human CPSF160-WDR33-CPSF30 complex bound to the PAS AAUAAA motif at 3.1 Angstrom resolution

Replaces:  6FBS

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Cleavage and polyadenylation specificity factor subunit 1	polymer	1446	161346.5	1	UniProt (Q10570) Pfam (PF10433) Pfam (PF03178) In PDB	Homo sapiens (Human)	Cleavage and polyadenylation specificity factor 160 kDa subunit,CPSF 160 kDa subunit
2	B	pre-mRNA 3' end processing protein WDR33	polymer	413	47448.9	1	UniProt (Q9C0J8) Pfam (PF00400) In PDB	Homo sapiens (Human)	WD repeat-containing protein 33,WD repeat-containing protein WDC146
3	C	Cleavage and polyadenylation specificity factor subunit 4	polymer	178	20422.9	1	UniProt (O95639) Pfam (PF00642) Pfam (PF15663) In PDB	Homo sapiens (Human)	Cleavage and polyadenylation specificity factor 30 kDa subunit,CPSF 30 kDa subunit,NS1 effector domain-binding protein 1,Neb-1,No arches homolog
4	D	RNA (5'-R(P*AP*AP*UP*AP*AP*AP*GP*G)-3')	polymer	10	3232.0	1		unidentified adenovirus
5	C	ZINC ION	non-polymer		65.4	3	Chemie (ZN)

Sequence modifications

A: 1 - 1443 (UniProt: Q10570)

PDB	External Database	Details
Ser -2	-	expression tag
Asn -1	-	expression tag
Ala 0	-	expression tag

B: 1 - 410 (UniProt: Q9C0J8)

PDB	External Database	Details
Ser -2	-	expression tag
Asn -1	-	expression tag
Ala 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	232450.3
Non-Polymers*	Number of molecules	3
	Total formula weight	196.2
All*	Total formula weight	232646.5

*Water molecules are not included.