6FSZ

Structure of the nuclear RNA exosome

This is a non-PDB format compatible entry.

Summary for 6FSZ

• Entry DOI: 10.2210/pdb6fsz/pdb
• EMDB information: 4301
• Descriptor: RNA (5'-R(P*AP*AP*AP*AP*UP*UP*UP*AP*AP*AP*UP*UP*UP*UP*UP*UP*UP*UP*UP*UP*UP*UP*U)-3'), Exosome complex component CSL4, Exosome complex exonuclease DIS3, ... (15 entities in total)
• Functional Keywords: rna exosome, ribosome, pre-ribosome, mtr4, helicase, rna
• Biological source: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast); More
• Total number of polymer chains: 15
• Total formula weight: 641388.94

Authors

Schuller, J.M.,Falk, S.,Conti, E. (deposition date: 2018-02-20, release date: 2018-03-21, Last modification date: 2024-05-15)

Primary citation

Schuller, J.M.,Falk, S.,Fromm, L.,Hurt, E.,Conti, E.
Structure of the nuclear exosome captured on a maturing preribosome.
Science, 360:219-222, 2018

PubMed Abstract: The RNA exosome complex processes and degrades a wide range of transcripts, including ribosomal RNAs (rRNAs). We used cryo-electron microscopy to visualize the yeast nuclear exosome holocomplex captured on a precursor large ribosomal subunit (pre-60) during 7-to-5.8 rRNA processing. The cofactors of the nuclear exosome are sandwiched between the ribonuclease core complex (Exo-10) and the remodeled "foot" structure of the pre-60 particle, which harbors the 5.8 rRNA precursor. The exosome-associated helicase Mtr4 recognizes the preribosomal substrate by docking to specific sites on the 25 rRNA, captures the 3' extension of the 5.8 rRNA, and channels it toward Exo-10. The structure elucidates how the exosome forms a structural and functional unit together with its massive pre-60 substrate to process rRNA during ribosome maturation.

PubMed: 29519915
DOI: 10.1126/science.aar5428

Experimental method

ELECTRON MICROSCOPY (4.6 Å)