6FRK

Structure of a prehandover mammalian ribosomal SRP and SRP receptor targeting complex

これはPDB形式変換不可エントリーです。

6FRK の概要

• エントリーDOI: 10.2210/pdb6frk/pdb
• EMDBエントリー: 4300
• 分子名称: Canis lupus familiaris RNA, 7SL, cytoplasmic 1 (RN7SL1), SRP RNA, Ribosomal protein uL3, Ribosomal protein uL4, ... (62 entities in total)
• 機能のキーワード: er membrane targeting ribosome signal recognition particle, translation
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast); 詳細
• 細胞内の位置: Cytoplasm : Q00004 P21262 P61010 P16255
• タンパク質・核酸の鎖数: 58
• 化学式量合計: 2504827.71

構造登録者

Kobayashi, K.,Jomaa, A.,Ban, N. (登録日: 2018-02-16, 公開日: 2018-03-28, 最終更新日: 2018-05-02)

主引用文献

Kobayashi, K.,Jomaa, A.,Lee, J.H.,Chandrasekar, S.,Boehringer, D.,Shan, S.O.,Ban, N.
Structure of a prehandover mammalian ribosomal SRP·SRP receptor targeting complex.
Science, 360:323-327, 2018

PubMed Abstract: Signal recognition particle (SRP) targets proteins to the endoplasmic reticulum (ER). SRP recognizes the ribosome synthesizing a signal sequence and delivers it to the SRP receptor (SR) on the ER membrane followed by the transfer of the signal sequence to the translocon. Here, we present the cryo-electron microscopy structure of the mammalian translating ribosome in complex with SRP and SR in a conformation preceding signal sequence handover. The structure visualizes all eukaryotic-specific SRP and SR proteins and reveals their roles in stabilizing this conformation by forming a large protein assembly at the distal site of SRP RNA. We provide biochemical evidence that the guanosine triphosphate hydrolysis of SRP·SR is delayed at this stage, possibly to provide a time window for signal sequence handover to the translocon.

PubMed: 29567807
DOI: 10.1126/science.aar7924

実験手法

ELECTRON MICROSCOPY (3.7 Å)