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6FJM

tubulin-Disorazole Z complex

Summary for 6FJM
Entry DOI10.2210/pdb6fjm/pdb
Related6FII 6FJF
DescriptorTubulin alpha-1B chain, Disorazole Z, PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER, ... (12 entities in total)
Functional Keywordscell cycle, tubulin fold, cytoskeleton, microtubule
Biological sourceRattus norvegicus (Rat)
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Total number of polymer chains6
Total formula weight265413.40
Authors
Menchon, G.,Prota, A.E.,Lucena Agell, D.,Bucher, P.,Jansen, R.,Irschik, H.,Mueller, R.,Paterson, I.,Diaz, J.F.,Altmann, K.-H.,Steinmetz, M.O. (deposition date: 2018-01-22, release date: 2018-05-30, Last modification date: 2024-01-17)
Primary citationMenchon, G.,Prota, A.E.,Lucena-Agell, D.,Bucher, P.,Jansen, R.,Irschik, H.,Muller, R.,Paterson, I.,Diaz, J.F.,Altmann, K.H.,Steinmetz, M.O.
A fluorescence anisotropy assay to discover and characterize ligands targeting the maytansine site of tubulin.
Nat Commun, 9:2106-2106, 2018
Cited by
PubMed Abstract: Microtubule-targeting agents (MTAs) like taxol and vinblastine are among the most successful chemotherapeutic drugs against cancer. Here, we describe a fluorescence anisotropy-based assay that specifically probes for ligands targeting the recently discovered maytansine site of tubulin. Using this assay, we have determined the dissociation constants of known maytansine site ligands, including the pharmacologically active degradation product of the clinical antibody-drug conjugate trastuzumab emtansine. In addition, we discovered that the two natural products spongistatin-1 and disorazole Z with established cellular potency bind to the maytansine site on β-tubulin. The high-resolution crystal structures of spongistatin-1 and disorazole Z in complex with tubulin allowed the definition of an additional sub-site adjacent to the pocket shared by all maytansine-site ligands, which could be exploitable as a distinct, separate target site for small molecules. Our study provides a basis for the discovery and development of next-generation MTAs for the treatment of cancer.
PubMed: 29844393
DOI: 10.1038/s41467-018-04535-8
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (2.1 Å)
Structure validation

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