6FCV
Structure of the human DDB1-CSA complex
Summary for 6FCV
Entry DOI | 10.2210/pdb6fcv/pdb |
Related | 4A11 |
Descriptor | DNA damage-binding protein 1, DNA excision repair protein ERCC-8 (2 entities in total) |
Functional Keywords | dna damage response protein, protein binding |
Biological source | Homo sapiens (Human) More |
Total number of polymer chains | 2 |
Total formula weight | 175695.63 |
Authors | Meulenbroek, E.M.,Pannu, N.S. (deposition date: 2017-12-21, release date: 2019-01-30, Last modification date: 2024-01-17) |
Primary citation | Pines, A.,Dijk, M.,Makowski, M.,Meulenbroek, E.M.,Vrouwe, M.G.,van der Weegen, Y.,Baltissen, M.,French, P.J.,van Royen, M.E.,Luijsterburg, M.S.,Mullenders, L.H.,Vermeulen, M.,Vermeulen, W.,Pannu, N.S.,van Attikum, H. TRiC controls transcription resumption after UV damage by regulating Cockayne syndrome protein A. Nat Commun, 9:1040-1040, 2018 Cited by PubMed Abstract: Transcription-blocking DNA lesions are removed by transcription-coupled nucleotide excision repair (TC-NER) to preserve cell viability. TC-NER is triggered by the stalling of RNA polymerase II at DNA lesions, leading to the recruitment of TC-NER-specific factors such as the CSA-DDB1-CUL4A-RBX1 cullin-RING ubiquitin ligase complex (CRL). Despite its vital role in TC-NER, little is known about the regulation of the CRL complex during TC-NER. Using conventional and cross-linking immunoprecipitations coupled to mass spectrometry, we uncover a stable interaction between CSA and the TRiC chaperonin. TRiC's binding to CSA ensures its stability and DDB1-dependent assembly into the CRL complex. Consequently, loss of TRiC leads to mislocalization and depletion of CSA, as well as impaired transcription recovery following UV damage, suggesting defects in TC-NER. Furthermore, Cockayne syndrome (CS)-causing mutations in CSA lead to increased TRiC binding and a failure to compose the CRL complex. Thus, we uncover CSA as a TRiC substrate and reveal that TRiC regulates CSA-dependent TC-NER and the development of CS. PubMed: 29531219DOI: 10.1038/s41467-018-03484-6 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (2.92 Å) |
Structure validation
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