6FCG

Crystal structure of an endo-laminarinase from Formosa Hel1_33_131

Summary for 6FCG

• Entry DOI: 10.2210/pdb6fcg/pdb
• Descriptor: Glycoside hydrolase, GH17 family, CALCIUM ION (3 entities in total)
• Functional Keywords: glycoside hydrolase, gh17, bacteria, laminarinase, hydrolase
• Biological source: Formosa sp. Hel1_33_131
• Total number of polymer chains: 6
• Total formula weight: 293799.45

Authors

Becker, S.,Robb, C.S.,Hehemann, J.H. (deposition date: 2017-12-20, release date: 2018-12-26, Last modification date: 2024-01-17)

Primary citation

Unfried, F.,Becker, S.,Robb, C.S.,Hehemann, J.H.,Markert, S.,Heiden, S.E.,Hinzke, T.,Becher, D.,Reintjes, G.,Kruger, K.,Kappelmann, L.,Hahnke, R.L.,Fischer, T.,Harder, J.,Teeling, H.,Fuchs, B.,Barbeyron, T.,Amann, R.I.,Schweder, T.
Adaptive mechanisms that provide competitive advantages to marine bacteroidetes during microalgal blooms.
ISME J, 12:2894-2906, 2018

PubMed Abstract: Polysaccharide degradation by heterotrophic microbes is a key process within Earth's carbon cycle. Here, we use environmental proteomics and metagenomics in combination with cultivation experiments and biochemical characterizations to investigate the molecular details of in situ polysaccharide degradation mechanisms during microalgal blooms. For this, we use laminarin as a model polysaccharide. Laminarin is a ubiquitous marine storage polymer of marine microalgae and is particularly abundant during phytoplankton blooms. In this study, we show that highly specialized bacterial strains of the Bacteroidetes phylum repeatedly reached high abundances during North Sea algal blooms and dominated laminarin turnover. These genomically streamlined bacteria of the genus Formosa have an expanded set of laminarin hydrolases and transporters that belonged to the most abundant proteins in the environmental samples. In vitro experiments with cultured isolates allowed us to determine the functions of in situ expressed key enzymes and to confirm their role in laminarin utilization. It is shown that laminarin consumption of Formosa spp. is paralleled by enhanced uptake of diatom-derived peptides. This study reveals that genome reduction, enzyme fusions, transporters, and enzyme expansion as well as a tight coupling of carbon and nitrogen metabolism provide the tools, which make Formosa spp. so competitive during microalgal blooms.

PubMed: 30061707
DOI: 10.1038/s41396-018-0243-5

Experimental method

X-RAY DIFFRACTION (2.6 Å)