6FCG
Crystal structure of an endo-laminarinase from Formosa Hel1_33_131
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, DESY BEAMLINE P11 |
Synchrotron site | PETRA III, DESY |
Beamline | P11 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-09-11 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.0332 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 93.336, 149.143, 107.217 |
Unit cell angles | 90.00, 103.35, 90.00 |
Refinement procedure
Resolution | 104.320 - 2.600 |
R-factor | 0.20629 |
Rwork | 0.205 |
R-free | 0.23881 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4wtp |
RMSD bond length | 0.013 |
RMSD bond angle | 1.668 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 104.320 | 2.650 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 87396 | 4421 |
<I/σ(I)> | 6.9 | 2.6 |
Completeness [%] | 99.7 | 99.9 |
Redundancy | 5.2 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.03M Magnesium Chloride 0.1M MOPS pH 7 9% PEG8000 15% Ethyleneglycol |