6FBE

KlenTaq DNA polymerase processing a modified primer - bearing the modification upstream at the third primer nucleotide.

Summary for 6FBE

DescriptorDNA polymerase I, thermostable, DNA (5'-D(*GP*AP*CP*CP*AP*CP*GP*CP*AP*(OH3)P*CP*C)-3'), DNA (5'-D(*AP*AP*AP*CP*GP*GP*GP*TP*GP*CP*GP*TP*GP*GP*TP*C)-3'), ... (8 entities in total)
Functional Keywordsdna polymerase, modified nucleotides, klentaq, klentaq dna polymerase, dna binding protein
Biological sourceThermus aquaticus
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Total number of polymer chains3
Total molecular weight70513.39
Authors
Kropp, H.M.,Diederichs, K.,Marx, A. (deposition date: 2017-12-19, release date: 2018-09-26, Last modification date: 2019-08-14)
Primary citation
Kropp, H.M.,Durr, S.L.,Peter, C.,Diederichs, K.,Marx, A.
Snapshots of a modified nucleotide moving through the confines of a DNA polymerase.
Proc. Natl. Acad. Sci. U.S.A., 115:9992-9997, 2018
PubMed: 30224478 (PDB entries with the same primary citation)
DOI: 10.1073/pnas.1811518115
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (1.589 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers 0.2031 0.2% 0.2% 3.7%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
Download full validation report