Crystal Structure of a Tailored I-CreI Homing Endonuclease Protein (3115 variant) in complex with its target DNA (Haemoglobin beta subunit gene) in the presence of Magnesium

Summary for 6FB1

DescriptorDNA endonuclease I-CreI, DNA (5'-D(*TP*CP*AP*GP*AP*CP*TP*TP*CP*TP*CP*CP*AP*C)-3'), DNA (5'-D(*TP*CP*TP*GP*AP*CP*TP*CP*CP*TP*GP*TP*GP*G)-3'), ... (8 entities in total)
Functional Keywordschlamydomonas reinhardtii, dna binding protein
Biological sourceChlamydomonas reinhardtii (Chlamydomonas smithii)
Total number of polymer chains6
Total molecular weight49947.05
Molina, R.,Prieto, J. (deposition date: 2017-12-18, release date: 2018-07-18)
Primary citation
Prieto, J.,Redondo, P.,Lopez-Mendez, B.,D'Abramo, M.,Merino, N.,Blanco, F.J.,Duchateau, P.,Montoya, G.,Molina, R.
Understanding the indirect DNA read-out specificity of I-CreI Meganuclease.
Sci Rep, 8:10286-10286, 2018
PubMed: 29980759 (PDB entries with the same primary citation)
DOI: 10.1038/s41598-018-28599-0
MImport into Mendeley
Experimental method

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.25460.3%0.7%0.3%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 6fb1
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Molmil generated image of 6fb1
rotated about x axis by 90°
Molmil generated image of 6fb1
rotated about y axis by 90°