6FB1
Crystal Structure of a Tailored I-CreI Homing Endonuclease Protein (3115 variant) in complex with its target DNA (Haemoglobin beta subunit gene) in the presence of Magnesium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-11-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.090, 66.600, 91.780 |
| Unit cell angles | 90.00, 96.69, 90.00 |
Refinement procedure
| Resolution | 33.985 - 3.024 |
| R-factor | 0.2103 |
| Rwork | 0.208 |
| R-free | 0.25180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g9z |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.696 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.600 | 3.150 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 18027 | |
| <I/σ(I)> | 5.4 | |
| Completeness [%] | 99.7 | |
| Redundancy | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M calcium acetate 0.1 M sodium acetate pH 4.6 35% (v/v) 1,2-propanediol |
