6FB0
Crystal Structure of a Tailored I-CreI Homing Endonuclease Protein (3115 variant) in complex with its target DNA (Haemoglobin beta subunit gene) in the presence of Calcium
Summary for 6FB0
Entry DOI | 10.2210/pdb6fb0/pdb |
Descriptor | DNA endonuclease I-CreI, DNA (5'-D(*TP*CP*AP*GP*AP*CP*TP*TP*CP*TP*CP*CP*AP*CP*AP*GP*GP*AP*GP*TP*CP*AP*GP*A)-3'), DNA (5'-D(*TP*CP*TP*GP*AP*CP*TP*CP*CP*TP*GP*TP*GP*GP*AP*GP*AP*AP*GP*TP*CP*TP*GP*A)-3'), ... (8 entities in total) |
Functional Keywords | chlamydomonas reinhardtii, dna binding protein |
Biological source | Chlamydomonas reinhardtii (Chlamydomonas smithii) More |
Total number of polymer chains | 4 |
Total formula weight | 50458.61 |
Authors | Molina, R.,Prieto, J. (deposition date: 2017-12-18, release date: 2018-07-18, Last modification date: 2024-01-17) |
Primary citation | Prieto, J.,Redondo, P.,Lopez-Mendez, B.,D'Abramo, M.,Merino, N.,Blanco, F.J.,Duchateau, P.,Montoya, G.,Molina, R. Understanding the indirect DNA read-out specificity of I-CreI Meganuclease. Sci Rep, 8:10286-10286, 2018 Cited by PubMed: 29980759DOI: 10.1038/s41598-018-28599-0 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (2.15 Å) |
Structure validation
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